rodent and rabbit assay data sheet
Mouse polyoma virus (POLY)
Test code:
S0100 -
Ultrasensitive qualitative detection of mouse polyoma virus by
real time PCR
S0100 is included on
P0029 - Mouse Essentials Panel
Mouse polyoma
virus (POLY) often originates in wild mice. Contaminated feed
and bedding are important sources of infection, as are
transplantable tumors and other biological specimens. The virus
is highly contagious and is shed in all secretions of an
infected animal, especially urine. Transmission is very rapid
between cagemates, cages and adjacent rooms in an animal
facility. Transplacental transmission has also been described.
Mice infected
with this virus usually have few if any clinical symptoms. Thus
many mice are potential carriers. The virus can induce tumor
formation, paralysis and wasting in nude and immunocompromised
mice.
Serological
detection of the virus is inadequate, especially in nude mice
and immunocompromised mice, which lack normal immunological
response. Furthermore, neonatal mice infected with the virus may
not be readily identified due to residual maternal antibodies.
Molecular detection by PCR overcomes these problems and provides
a highly sensitive and specific detection technique.
Utilities:
-
Help confirm the disease causing agent
-
Shorten the time required to confirm a clinical
diagnosis of mouse polyoma virus
-
Help ensure that vivariums are free of mouse polyoma virus
-
Early prevention of spread of mouse polyoma virus among
a vivarium
-
Minimize personnel exposure to this virus
-
Safety monitoring of biological products that derive
from mice
Specimen requirements:
0.2
ml urine, or 0.2 ml whole blood in EDTA (purple top) tube, or 0.2 ml fresh, frozen or fixed tissue,
or 0.2 ml cell culture.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See
shipping instructions
for more information.
Turnaround time:
2 business days
Methodology:
Qualitative
real time polymerase chain reaction
Normal range: Nondetected
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