Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

For wild rodent infestations, remediation and environmental monitoring, use our Rodent Infestation PCR Panel

* * *

Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

BXV-1 virus

Campylobacter

Chapparvovirus

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Leptospira

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse kidney parvovirus (MKV or MKPV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit coronavirus

Rabbit fibroma virus

Rabbit hemorrhagic disease virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi/ paraluiscuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


BXV-1 PCR test for cell lines and mice
rodent and rabbit assay data sheet

BXV-1 virus

Test code: S0241 - Ultrasensitive qualitative detection of BXV1 virus proviral DNA by real time polymerase chain reaction

BXV-1 (also known as XMV43 or B10 xenotropic virus 1), is a xenotropic endogenous virus found in certain strains of mice, especially common inbred strains and some Japanese Mus molossinus mice. Reactivation of this endogenous gammaretrovirus is possible after chemical or immunological stimulation.

The impact of this virus in biomedical research and biological production is significant. In one study (Sfanos et al., 2011), it was shown that 58 of the 60 human cancer cell lines used in anticancer drug screens and maintained at NCI-Frederick (NCI-60) were contaminated with this retrovirus. Some commonly used prostate cancer cell lines, such as CWR22Rv1, LAPC4 and VCaP, were also shown to be contaminated. It is suspected that many of these common human cell lines are contaminated as a result of passing the primary human tumors through immunocompromised mice, which is a common method of establishing permanent cell lines.

Retrovirus infection of these cell lines can alter their biological properties, rendering drug screening results potentially misleading. Furthermore, vaccine production often uses cell lines which can potentially be contaminated with this retrovirus; thus, screening of these cell lines for this virus is important to ensure vaccine safety.

Detection of proviral DNA of this virus is often performed by PCR, which is highly specific and sensitive.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of BXV-1 infection
  • Detect the presence of BXV-1 virus in cell lines
  • Help ensure that rodent colonies are free of this virus
  • Early prevention of spread of this virus among a colony
  • Minimize human exposure to this virus
  • Safety monitoring of biological products that derive from rodents

References:
Sfanos, K.S., Aloia, A.L., Hicks, J.L., Esopi, D.M., Steranka, J.P., Shao, W., et al. (2011) Identification of replication competent murine gammaretroviruses in commonly used prostate cancer cell lines. PLoS ONE 6(6): e20874.

Specimen requirements: 0.2 ml cell culture or vaccine, or 0.2 ml EDTA whole blood, or 0.2 ml fresh or frozen tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

2003-2021 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880