rodent and rabbit assay data sheet
S0241 - Ultrasensitive qualitative detection of BXV1 virus
proviral DNA by
real time polymerase chain
BXV-1 (also known as XMV43 or B10 xenotropic virus 1), is a xenotropic
endogenous virus found in certain strains of mice, especially
common inbred strains and some Japanese
Mus molossinus mice.
Reactivation of this endogenous gammaretrovirus is possible
after chemical or immunological stimulation.
The impact of this virus in biomedical research and biological production
is significant. In one study (Sfanos et al., 2011), it was shown
that 58 of the 60 human cancer cell lines used in anticancer
drug screens and maintained at NCI-Frederick (NCI-60) were
contaminated with this retrovirus. Some commonly used prostate
cancer cell lines, such as CWR22Rv1, LAPC4 and VCaP, were also
shown to be contaminated. It is suspected that many of these
common human cell lines are contaminated as a result of passing
the primary human tumors through immunocompromised mice, which
is a common method of establishing permanent cell lines.
Retrovirus infection of these cell lines can alter their biological
properties, rendering drug screening results potentially
misleading. Furthermore, vaccine production often uses cell
lines which can potentially be contaminated with this
retrovirus; thus, screening of these cell lines for this virus
is important to ensure vaccine safety.
Detection of proviral DNA of this virus is often performed by PCR, which
is highly specific and sensitive.
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of BXV-1 infection
Detect the presence of BXV-1 virus in cell lines
Help ensure that rodent colonies are free of this virus
Early prevention of spread of this virus among a colony
Minimize human exposure to this virus
Safety monitoring of biological products that derive
Sfanos, K.S., Aloia, A.L., Hicks, J.L., Esopi, D.M., Steranka, J.P.,
Shao, W., et al. (2011) Identification of replication competent
murine gammaretroviruses in commonly used prostate cancer cell
lines. PLoS ONE 6(6): e20874.
0.2 ml cell culture or vaccine, or 0.2 ml EDTA whole blood, or 0.2 ml fresh or frozen
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
real time PCR