rodent and rabbit assay data sheet
Human adenovirus PCR screen
S0177 - Ultrasensitive qualitative detection but not
differentiation of most reported human adenoviruses by real time
polymerase chain reaction
Adenoviruses, members of the family Adenoviridae, are
nonenveloped viruses with an icosahedral nucleocapsid containing
a double stranded DNA genome. These viruses survive well in the
environment and can infect both humans and animals.
In humans, more than 50 serotypes of adenovirus have been
identified. Human adenoviruses commonly infect the respiratory
and GI tracts, the eye, and various other tissues. Most
infections are asymptomatic, but long-term persistent infections
are possible, and are a major concern to immunocompromised
patients. Adenoviruses are known to be oncogenic in rodents but
not in humans. They can be transmitted via direct inoculation to
the conjunctiva, the fecal-oral route, aerosolized droplets, or
through exposure to infected tissue or blood.
Serological diagnosis of adenoviral infection may not be
suitable because of anti-adenovirus antibodies resulting from
previous exposures. Culture detection of these viruses may take
up to three weeks. Molecular detection by polymerase chain
reaction is highly sensitive and specific, and is a useful
detection method for these viruses (Al-Siyabi et al., 2013).
• Help confirm the disease causing agent
• Help ensure that animal populations are free of
• Early prevention of spread of these
viruses among a group of animals
• Minimize human exposure
to these viruses
• Safety monitoring of biological products
and vaccines that derive from susceptible animals
Al-Siyabi, T., Binkhamis,
K., Wilcox, M., Wong, S., Pabbaraju, K., Tellier, R. and
Hatchette, T.F. (2013) A cost effective real-time PCR for the
detection of adenovirus from viral swabs. Virol. J. 10:184-195.
Specimen requirements: 0.2 ml whole blood
in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml
cerebrospinal fluid, or 0.2 ml feces or 0.2 ml urine, or fecal
swab, or nasopharyngeal swab, or conjunctival swab.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See shipping instructions for
Turnaround time: 2 business days
Methodology: Qualitative real time PCR
Normal range: Nondetected