Ruminating about hoofstock issues?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel



Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi



Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever





Coxiella burnetii


Cryptosporidium serpentis

Cryptosporidium varanii (formerly saurophilum)

Delftia acidovorans

E. coli O157:H7

E. coli panel





Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus




Hepatitis E


Japanese encephalitis

Johne's disease

Kangaroo herpesviruses


Lawsonia intracellularis




Listeria monocytogenes

Lizard quarantine panel

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus


Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Ophidiomyces ophiodiicola

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia


Q fever



Reovirus screen


Rift Valley fever



Sarcocystis neurona

Snake fungal disease

Snake quarantine panel

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum


Trypanosoma cruzi

Trypanosoma evansi


Valley Fever

Vesicular stomatitis


West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis

Yersinia pseudotuberculosis PCR test

wildlife and zoo assay data sheet

Yersinia pseudotuberculosis

Test code:
B0062 - Qualitative ultrasensitive detection of Yersinia pseudotuberculosis bacteria by real time polymerase chain reaction.

Yersinia pseudotuberculosis is a gram-negative bacterium which primarily causes disease in animals, although humans may also be infected through food contamination. Infection with this pathogen can cause tuberculosis-like symptoms, including localized tissue necrosis and granulomas in the spleen, liver, and lymph node.

The bacteria occur widely in various host species, including dogs, cats, horses, cattle, rabbits, deer, rodents, and birds. Documented cases of occupational exposure to Y. pseudotuberculosis include butchers working in abattoirs slaughtering swine. Outbreaks of Y. pseudotuberculosis in animal facilities have occasionally been reported, including in primate facilities (Bronson et al., 1972; Iwata et al., 2008).

Successfully culturing Y. pseudotuberculosis is very difficult, and serologic tests in many cases require serial testing on multiple serum samples taken at several time points. Molecular detection by PCR offers a rapid, sensitive and specific method for detecting Y. pseudotuberculosis.


  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Yersinia pseudotuberculosis infection
  • Help ensure animal groups and populations are free of Y. pseudotuberculosis
  • Early prevention of spread of Y. pseudotuberculosis among a population
  • Minimize human exposure to Y. pseudotuberculosis
  • Safety monitoring of biological products and vaccines that derive from susceptible animals

Bronson, R.T., May, B.D. and Ruebner, B.H. (1972) An Outbreak of Infection by Yersinia pseudotuberculosis in Nonhuman Primates. Am. J. Pathol. 69: 289–308.
Iwata, T., Une, Y., Okatani, A.T., Kato, Y., Nakadai, A., Lee, K., Watanabe, M., Taniguchi, T., Elhelaly, A.E., Hirota, Y. and Hayashidani, H. (2008) Virulence characteristics of Yersinia pseudotuberculosis isolated from breeding monkeys in Japan. Vet Microbiol. 129:404-409.

Specimen requirement:  Rectal swab, or cloacal swab, or 0.2 ml feces, or 0.2 ml fresh, frozen or fixed tissue, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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