wildlife and zoo assay data sheet
Vesicular stomatitis virus (VSV)
S0068 - Ultrasensitive qualitative detection of vesicular
stomatitis virus by reverse transcription coupled real time polymerase
chain reaction. This assay
detects and differentiates
the New Jersey and Indiana serotypes of the vesicular stomatitis
stomatitis (VS) is a disease of horses, cattle, swine, sheep,
goats and deer caused by related viruses in the genus
Vesiculovirus of the family Rhabdoviridae. The viral genome is
11 kb and codes for five main proteins: the nucleocapsid (N),
phosphoprotein (P), matrix (M), glycoprotein(G), and polymerase
(L). Serologically, VS viruses have been classified into two
main serotypes: New Jersey (NJ) and Indiana (IN). In addition to
the classic VSV-IN (IN1), two additional subtypes, Cocal virus
(VSV-IN2) and Alagoas virus (VSV-IN3), have been described.
prevalent in northern South America, throughout Central America,
and at one site in the United States: Ossabaw Island, Georgia.
However, outbreaks of VS have been reported in the western
United States, northern Mexico, and temperate regions of South
America. The disease can cause blisters on an animal's mouth,
teats or hooves. Clinical outbreaks of VS in livestock can
result in significant economic impact on producers and extensive
regulatory responses by governmental agencies, including trade
restrictions, market closures, and quarantines. Such measures
are needed because of the clinical similarities between VS and
foot-and-mouth disease and other foreign vesicular diseases in
Help confirm the disease causing agent
Distinguish between VS and other vesicular diseases
Shorten the time required to confirm a clinical
diagnosis of VS infection
Help ensure that animal herds and populations are free of VS
Early prevention of spread of VS
Minimize human exposure to the virus
Magnuson, R.J., Triantis, J., Rodriguez, L.L., Perkins, A.,
Meredith, C.O., Beaty, B., McCluskey, B., and Salman M. (2003) A
single-tube multiplex reverse transcription-polymerase chain
reaction for detection and differentiation of vesicular
stomatitis Indiana 1 and New Jersey viruses in insects. J. Vet.
Diagn. Invest. 15:561-567.
Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top)
tube, or lesion swab or nasopharyngeal swab, or 0.2 ml nasal
wash, or 0.2 ml fresh or frozen tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
Qualitative reverse transcription coupled real time PCR