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rodent and rabbit assay data sheet
Mycoplasma
Test
codes:
B0009
-
Ultrasensitive Mycoplasma screen by real time PCR. This
screen detects but does not differentiate M. arginini, M.
fermentans, M. hominis, M. hyorhinis, M. orale, M. pirum, M.
salivarium, M. agassizii, M. cynos and others. This screen does
not detect M. pneumoniae or M. pulmonis.
B0041
- Ultrasensitive qualitative detection of
Mycoplasma pulmonis
by real time polymerase chain reaction
B0041 is included on P0029 -
Mouse Essentials Panel
Murine
respiratory mycoplasmosis in mice and rats is caused by
Mycoplasma pulmonis.
In addition to exhibiting high morbidity and reduced birth rate,
mice and rats infected with
M. pulmonis often develop imperceptible infections.
These can cause problems in research studies using these
animals, as physiological mechanisms and the immune system may
be affected. Experimental results obtained with infected animals
can be misleading.
Diagnosis of
M. pulmonis
infection can be done using serological methods such as
microbial isolation or enzyme linked immunosorbent assay (ELISA)
(Cassell et al., 1981). However, in vitro isolation is
time-consuming, and serological methods often give incorrect
results due to cross-reactivity between different species of
rodent mycoplasmas (Cassell et al., 1981; Davis et al., 1987;
Davidson et al., 1994). Serological assays also lack sensitivity
because a low level of antibody -- early in an infection or in
immunodeficient animals -- may not be detected.
Detection of
this pathogen by polymerase chain reaction is especially
important because it is very sensitive and specific. The result
will not be compromised if the infection is at an early stage or
the animal is immunodeficient.
Utilities:
-
Confirm the disease causing agent
-
Shorten the time required to confirm a clinical
diagnosis of M. pulmonis
infection.
-
Ensure that rodent colonies are free of
M. pulmonis
-
Early prevention of spread of
M. pulmonis
among a colony
-
Minimize personnel exposure to
M. pulmonis
-
Safety monitoring of biological products that derive
from rodents
References:
Cassell, G.H., Lindsey, J.R., Davis, J.K., Davidson, M.K.,
Brown, M.B. and Mayo, J.G. (1981) Detection of natural
Mycoplasma pulmonis infection in rats and mice by an enzyme
linked immunosorbent assay (ELISA). Lab Anim Sci 31: 676–682.
Davis, J.K., Cassell, G.H., Gambill, G., Cox, N., Watson, H. and
Davidson, M. (1987) Diagnosis of murine mycoplasmal infections
by enzyme-linked immunosorbent assay (ELISA). Isr J Med Sci 23:
717–722.
Davidson, M.K, Davis, J.K., Gambill, G.P., Cassell,
G.H. and Lindsey, J.P. (1994) Mycoplasmas of laboratory
rodentsMycoplasmas in Animals: Laboratory Diagnosis, Iowa State
University Press, Ames p. 97–133.
Specimen requirements:
B0009
- 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top)
tube, or 0.2 ml fresh, frozen or fixed tissue, or nasal swab, or
vaginal swab, or 0.5 ml bacterial culture.
B0041
- Nasal swab.
For specimen
types other than those listed here, please call to confirm
specimen acceptability and shipping instructions.
For all
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
Turnaround time:
2 business days
Methodology:
Qualitative
real time PCR
Normal range:
Nondetected
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