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rodent and rabbit assay data sheet
Sendai virus (SEND)
Test code:
S0099 -
Ultrasensitive qualitative detection of Sendai virus by reverse
transcription coupled real time PCR
S0099 is included on
P0029 - Mouse Essentials Panel
Sendai virus
(SEND) is an RNA paramyxovirus of the parainfluenza type 1
group. Mice infected with this virus develop labored breathing
and decreased fecundity. In DBA/2 and immune deficient mice, the
infection is almost always fatal. This virus immunosuppresses
affected mice and predisposes them to secondary bacterial
infections.
Generally, no
clinical signs are observed in mice in endemically infected
colonies. Thus many mice may be carriers of the virus. In
clinically apparent infections, signs are variable but may
include chattering, mild respiratory distress to labored
breathing, and decreased fecundity in adults. Deaths (possibly
whole litters) in neonates and suckling mice can occur due to
the weak immune defense at this age. In young adult mice, poor
growth is common.
Direct contact
is the primary means of viral spread. The virus is not
environmentally stable, but can be transmitted by fomites
because of the quantities of virus excreted from infected mice.
Serology
testing for these viruses has been used extensively to test for
the immune status of mice. However, this testing approach is
often inadequate to control the spread of the virus, largely
because it takes a long time to generate a detectable
serological response when the mice are exposed to this virus.
Secondly, the sensitivity of serological testing is often not
comparable to molecular detection by PCR. Thirdly, serological
testing cannot be applied to immunosuppressed mice, such as nude
mice. Fourthly, swabs can be conveniently used by PCR to detect
the presence of Sendai virus.
Utilities:
-
Confirm the disease causing agent
-
Shorten the time required to confirm a clinical
diagnosis of Sendai virus
-
Ensure that vivariums are free of Sendai virus
-
Early prevention of spread of Sendai virus among a
vivarium
-
Minimize personnel exposure to this virus
-
Safety monitoring of biological products that derive
from mice
Specimen requirements:
Nasopharyngeal swab, or 0.2 ml fresh or frozen tracheal or lung
tissue.
For specimen
types other than those listed here, please call to confirm
specimen acceptability and shipping instructions.
For all
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See
shipping instructions
for more information.
Turnaround time:
2 business days
Methodology:
Qualitative
reverse transcription coupled real time polymerase chain
reaction
Normal range: Nondetected
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