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rodent and rabbit assay data sheet
Mouse norovirus (MNV)
Test code:
S0138 - Ultrasensitive qualitative detection of mouse norovirus by
reverse transcription coupled real time PCR
S0138
is included on P0029 - Mouse
Essentials Panel and on
P0032 Mouse Minipanel
Noroviruses are a group of genetically related and
antigenically diverse viruses that can infect both humans and
animals. They are caliciviruses and carry positive
single-stranded RNA. These viruses are resistant to organic
solvents and mild detergents and hence can survive in the
environment for some time. Transmission occurs by ingestion of
material contaminated by infected feces. These viruses infect
and replicate in dendritic cells of the gut and in macrophage
cells.
Mouse norovirus
was first identified in 2003. Immunocompetent mice infected with
the virus can develop transient infection with a short duration
of fecal shedding of the virus. Mice that are immunocompromised,
including some knockout strains, can develop lethal infection.
Sharing of mice between animal facilities can significantly
increase the risk of spreading the infection.
Serologic
assays can be used to detect exposure of mice to the virus, but
it usually takes a long time for detectable serological changes
to occur.
However, even recently-infected mice will shed the virus in
feces, allowing viral RNA to be readily detected by reverse
transcription coupled real time PCR (Kelmenson et al., 2009).
PCR detection is highly sensitive, specific and rapid;
this technique provides a valuable tool for screening mice being
moved between facilities.
Utilities:
-
Confirm the
disease causing agent
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Shorten the time required to confirm a clinical
diagnosis of norovirus
-
Ensure that vivariums are free of norovirus
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Early prevention of spread of norovirus among a vivarium
-
Minimize personnel exposure to this virus
-
Safety monitoring of biological products that derive
from mice
References:
Kelmenson,
J.A., Pomerleau, D.P., Griffey, S., Zhang, W., Karolak, M.J. and
Fahey, J.R. (2009) Kinetics of transmission, infectivity, and
genome stability of two novel mouse norovirus isolates in
breeding mice. Comp. Med. 59:27-36
Specimen requirements:
1 fecal pellet.
For specimen
types other than those listed here, please call to confirm
specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions
for more information.
Turnaround time:
2 business days
Methodology:
Qualitative
reverse transcription coupled real time polymerase chain
reaction
Normal range: Nondetected
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