Encephalomyocarditis PCR test for rodents

Encephalomyocarditis virus (EMCV) is a single stranded picornavirus belonging to the cardiovirus genus that infects many animal species including pigs, rodents, cattle, elephants, raccoons , marsupials, baboons, macaques, chimpanzees and humans . Rats and mice are the natural hosts of the virus, but pigs are the most commonly and severely infected domestic animals. The ability of this virus to cause interspecies infections had led to numerous outbreaks in zoos in Australia and the United States (Reddacliff et al., 1997; Wells and Gutter, 1989). These outbreaks involved multiple animal species including lemurs, squirrels, macaques, mandrills, chimpanzees, hippopotami, kangaroos and possibly humans. Humans infected with this virus may have symptoms including fever, neck stiffness, lethargy, delirium, headaches, or vomiting (Gajdusek, 1955; Murname, 1981). In recent years, there has been renewed interest in this virus, especially in pig-to-human transmission, because of advances in xenotransplantation as a means of overcoming the acute shortage of transplantation tissues and organs for humans.

In the past, diagnosis of EMCV was based on virus isolation and identification. This method is time-consuming and the virus is difficult to isolate from infected animals. Experimental EMCV infection in pigs showed that virus could no longer be isolated after 3 days post-infection (Foni et al., 1992), but the virus may continually persist for a long period in infected pigs without any clinical signs (Billinis et al., 1999). Confirmation of this pathogen has relied upon the development of circulating antibody, but this diagnostic approach is not reliable because a recent study in pigs has shown that some infected pigs may not develop antibodies against EMCV (Brewer et al., 2001).

EMCV detection by PCR is a rapid, sensitive and specific method for the diagnosis of this infection. PCR methodology can reduce the frequency of false negative diagnoses of this virus.

Utilities:

Confirm the disease causing agent
Shorten the time required to confirm a clinical diagnosis of EMCV infection.
Ensure that rodent colonies are free of this virus
Early prevention of spread of EMCV among a colony
Minimize human exposure to this virus
Safety monitoring of biological products that derive from rodents

References:
Reddacliff, L. A., P. D. Kirland, W. J. Hartley, and R. L. Reece (1997). Encephalomyocarditis virus infections in an Australian zoo. J. Zoo Wildl. Med. 28:153-157.
Wells, S. K., and A. E. Gutter. (1989). Encephalomyocarditis virus: epizootic in a zoological collection. J. Zoo Wildl. Med. 20:291-296.
Gajdusek, C. (1955). Encephalomyocarditis infection in childhood. Pediatrics 16:819.
Murname, T. G. 1981. Encephalomyocarditis, p. 137-147. In G. W. Beran (ed.), CRC handbook series in zoonoses, section B, vol. 2. Viral zoonoses. CRC Press, Boca Raton, Fla.
Foni, E., Barigazzi, G., Sidoli, L., Marcato, P.S., Sarli, G., Della Salda, L. and Spinaci , M. (1993). Experimental Encephalomyocarditis virus infection in pigs. J. Vet. Med. 40:347–352.
Billinis, C., Paschaleri-Papadopoulou, E., Psychas, V., Vlemmas, J., Leontides, S., Koumbati, M., Kyriakis, S.C. and Papadopoulos , O. (1999) Persistence of Encephalomyocarditis virus (EMCV) infection in piglets. Vet. Microbiol. 70:171–177.
Brewer, L.A., Lwamba, H.C., Murtaugh, M.P., Palmenberg, A.C., Brown, C. and Njenga, M.K.(2001) Porcine encephalomyocarditis virus persists in pig myocardium and infects human myocardial cells. J.Virol. 75:11621-11629

Specimen requirements: 0.1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.1 ml plasma, serum or tissue, shipped overnight at room temperature; or 0.1 ml frozen plasma, serum or tissue, shipped frozen.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription PCR

Normal range: Nondetected