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rodent and rabbit assay data sheet
Mouse adenovirus 1 and 2 (MAD-1 and MAD-2)
Test codes:
S0146
- Ultrasensitive qualitative detection of mouse adenovirus 1
(MAD-1) by real time PCR
S0147
- Ultrasensitive qualitative detection of mouse adenovirus 2
(MAD-2) by real time PCR
S0146 and S0147 are included on
P0029 - Mouse Essentials Panel
Mouse
adenoviruses are DNA viruses; there are two strains, group 1
(MAD-1) and group 2 (MAD-2). These viruses infect both mice and
rats.
Infection with
these viruses does not cause clinical disease in adult rodents,
and there are no pathologic lesions associated with infections
of MAD-1 in adult mice. However, MAD-1 infection can produce a
lethal disease in newborn or suckling mice characterized by
infectious virus and viral lesions in multiple organs. Viral
inclusions in intestinal mucosa are associated with MAD-2
infections.
Infection of
mice or rats with adenoviruses can alter their normal immune
response and thereby skew experimental data. For example,
infection with MAD-1 can produce extensive persistent lesions in
the kidneys of adult mice and render them more susceptible to
experimental Escherichia
coli-induced pyelonephritis. Mouse adenovirus infection has
also been shown to accelerate experimental scrapie infection in
mice. Although mouse adenoviral infection is usually subclinical
in immunocompetent mice, wasting may result in nude mice.
Serological
detection may not be useful because many mice and rats may have
prior exposure to these viruses. Hemagglutination methodology is
also not suitable for mouse adenovirus detection because these
viruses simply will not hemagglutinate. Molecular detection of
adenoviruses is rapid, specific and sensitive to detect the
presence of the viruses.
Utilities:
-
Confirm the disease causing agent
-
Shorten the time required to confirm a clinical
diagnosis of mouse adenoviruses
-
Ensure that vivariums are free of mouse adenoviruses
-
Early prevention of spread of mouse adenoviruses among a
vivarium
-
Minimize personnel exposure to these viruses
-
Safety monitoring of biological products that derive
from mice
References:
Implications of infectious agents on results of animal
experiments. Report of the Working Group on Hygiene of the
Gesellschaft für Versuchstierkunde--Society for Laboratory
Animal Science (GV-SOLAS). Lab. Animal. 1999; 33 Suppl 1:S39-87.
Specimen requirements:
0.2 ml
whole blood in EDTA (purple top) or ACD (yellow top) tube; or
0.2 ml tissue.
For specimen
types other than those listed here, please call to confirm
specimen acceptability and shipping instructions.
For all
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See
shipping instructions
for more information.
Turnaround time:
2 business days
Methodology:
Qualitative
real time polymerase chain reaction
Normal range: Nondetected
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