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rodent and rabbit assay data sheet
Yersinia pseudotuberculosis
Test code: B0062
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Ultrasensitive qualitative detection of
Yersinia pseudotuberculosis
by real time
PCR.
Yersinia pseudotuberculosis
is a gram-negative bacterium which primarily causes disease in
animals, although humans may also be infected through food
contamination. Infection with this pathogen
can cause tuberculosis-like symptoms, including localized tissue
necrosis and granulomas in the spleen, liver, and lymph node.
The bacteria
occur widely in various host species, including dogs, cats,
horses, cattle, rabbits, deer, rodents, and birds. Documented
cases of occupational exposure to
Y. pseudotuberculosis include butchers working in abattoirs
slaughtering swine. Outbreaks of
Y. pseudotuberculosis
in animal facilities have occasionally been reported, including
in primate facilities (Bronson et al., 1972; Iwata et al.,
2008).
Successfully
culturing Y.
pseudotuberculosis is very difficult, and serologic tests in
many cases require serial testing on multiple serum samples
taken at several time points. Molecular detection by PCR offers
a rapid, sensitive and specific method for detecting
Y. pseudotuberculosis.
Utilities:
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Confirm the disease causing agent
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Shorten the time required to confirm a clinical
diagnosis of
Yersinia pseudotuberculosis
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Ensure that animal facilities are free of
Yersinia pseudotuberculosis
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Early prevention of spread of
Yersinia pseudotuberculosis among a facility
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Minimize human exposure to
Yersinia pseudotuberculosis
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Safety monitoring of biological products that derive
from susceptible animals
References:
Bronson, R.T., May, B.D. and Ruebner, B.H. (1972) An Outbreak of
Infection by Yersinia pseudotuberculosis in Nonhuman Primates. Am. J. Pathol. 69:
289–308.
Iwata, T., Une, Y., Okatani, A.T., Kato, Y., Nakadai,
A., Lee, K., Watanabe, M., Taniguchi, T., Elhelaly, A.E., Hirota,
Y. and Hayashidani, H. (2008) Virulence characteristics of
Yersinia pseudotuberculosis isolated from breeding monkeys in
Japan. Vet Microbiol. 129:404-409.
Specimen requirements:
Fecal pellet, or 0.2 ml whole blood in EDTA (purple top) or ACD
(yellow top) tube.
For specimen
types other than those listed here, please call to confirm
specimen acceptability and shipping instructions.
For all
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See
shipping instructions
for more information.
Turnaround time:
2 business days
Methodology:
Qualitative
polymerase chain reaction
Normal range: Nondetected
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