Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Leptospira

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit coronavirus

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi/ paraluiscuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Rabbit coronavirus PCR test

rodent and rabbit assay data sheet

Rabbit coronavirus

Test code: S0231 - Ultrasensitive qualitative detection of rabbit coronavirus by real time PCR

 

Coronaviruses (CoVs) are found in a wide variety of animals, in which they can cause respiratory, enteric, hepatic, and neurological diseases of various severities. Recent taxonomy revision has placed coronaviruses into three genera, alphacoronavirus, betacoronavirus, and gammacoronavirus, replacing the traditional group 1, 2, and 3 CoVs. Recently, novel CoVs have been discovered in birds and pigs, giving rise to a new genus, deltacoronavirus.

Due to their unique mechanism of viral replication, CoVs have a high frequency of recombination and because of their high mutation rates as result of RNA replication, they can adapt to new hosts and ecological niches easily.

Rabbit CoVs belong to betacoronavirus subgroup A. Rabbit CoVs have been observed in feces of clinically healthy adult rabbits and these sub-clinically infected rabbits can shed the virus for a long time. The virus is usually transmitted by fecal-oral route. This viral infection has been reported in some cases to cause acute intestinal disease with diarrhea and high mortality in young rabbits. However, more commonly it causes only mild diarrhea and is often only clinically significant with concurrent E. coli infection.

Culture detection of this virus is usually very slow and not sensitive. Immunological detection is hampered by the fact that prior exposure to other coronaviruses can result in cross-reactivity. Molecular detection by polymerase chain reaction (PCR) is fast, sensitive and specific (Van Elden et al., 2004).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of rabbit coronavirus
  • Help ensure that rabbit colonies or populations are free of this virus
  • Early prevention of spread of this virus among a population or in a geographic area
  • Minimize human exposure to this virus
  • Safety monitoring of biological products that derive from rabbits

References:
Van Elden, L.J.R. et al (2004) Frequent detection of human coronaviruses in clinical specimens from patients with respiratory tract infection by use of a novel real-time reverse-transcriptase polymerase chain reaction. J. Infect. Dis., 189: 652–657.

Specimen requirements: 0.2 ml feces, or fecal or rectal swab, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time polymerase chain reaction

Normal range: Nondetected

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