Zoologix

equine assay menu
Use this alphabetical index to click directly to your assay of interest.
Once there, click on the assay name to view its data sheet.

African horse sickness
Babesia equi
Babesia caballi
Breeding panel
Borrelia burgdorferi
Burkholderia mallei
Burkholderia pseudomallei
Clostridium difficile
Clostridium species screen
Contagious equine metritis
Diarrhea panel
Dourine
Eastern equine encephalitis
E. coli O157:H7
E. coli panel
Ehrlichia risticii
Equine arteritis virus

Equine herpesvirus type I
Equine herpesvirus type II
Equine herpesvirus type III
Equine herpesvirus type IV
Equine herpesvirus type V
Equine infectious anemia
Equine monocytic ehrlichiosis
Equine piroplasmosis
Equine protozoal myeloencephalitis
GI panel
Giardia
Glanders
Helicobacter heilmannii
Helicobacter pylori
Helicobacter species screen and species ID
Influenza

Japanese encephalitis
Lawsonia intracellularis
Leptospira
Lyme disease
Melioidosis
Neorickettsia risticii
Neospora hughesi
Neospora caninum
Neurological panel
Potomac horse fever
Respiratory panel
Rhodococcus equi
Rotavirus
Salmonella
Sarcocystis neurona
St. Louis encephalitis
Strangles

Streptococcus equi, subspecies equi
Streptococcus equi, subspecies zooepidemicus
Streptococcus pneumoniae
Strongyles
Surra
Tapeworms
Taylorella equigenitalis
Toxoplasma gondii
Treponema pallidum
Trichomonas
Trypanosoma equiperdum
Trypanosoma evansi
Venezuelan equine encephalitis
Vesicular stomatitis
West Nile virus
Western equine encephalitis
Yersinia pseudotuberculosis

Assay name with links to assay data sheets	Assay code	Preferred sample type*	Turnaround time	Methodology	Comments
Streptococcus pneumoniae	B0001	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or serum, pleural fluid, CSF or bacterial culture, shipped overnight at room temperature.	2 business days	Qualitative PCR	Normal range: Nondetected
Borrelia burgdorferi (Lyme disease)	B0004	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or synovial fluid, CSF, urine, tissue or tick, shipped overnight at room temperature.	2 business days	Qualitative PCR	Normal range: Nondetected
Treponema pallidum	B0010	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or serum, CSF, vaginal swab, culture or tissue, shipped overnight at room temperature.	2 business days	Qualitative PCR	Normal range: Nondetected
Contagious equine metritis - ultrasensitive	B0017	1 ml semen, amniotic fluid, CSF, vaginal swab or culture, shipped overnight at room temperature.	2 business days	Qualitative real time PCR	Normal range: Nondetected.
Streptococcus equi (strangles) - ultrasensitive	B0019	Nasopharyngeal swab, or lymph node abscess swab, or gutteral pouch swab, or tissue, or 1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, shipped overnight at room temperature.	2 business days	Qualitative real time PCR	Normal range: Nondetected. This assay utilizes a real time PCR procedure to achieve a highly sensitive detection and differentiation of subspecies equi and subspecies zooepidemicus.
Helicobacter pylori - ultrasensitive	B0021	1 ml gastric lavage or feces or tissue shipped overnight at room temperature; or tissue shipped frozen.	2 business days	Qualitative real time PCR	Normal range: Nondetected.
Helicobacter heilmannii - ultrasensitive	B0023	1 ml gastric lavage or feces or tissue shipped overnight at room temperature; or tissue shipped frozen.	2 business days	Qualitative real time PCR	Normal range: Nondetected.
Burkholderia mallei and pseudomallei ("glanders") - ultrasensitive	B0025	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or sputum, pus or bacterial subculture, shipped overnight at room temperature.	2 business days	Qualitative real time PCR	Normal range: Nondetected. This assay utilizes a nested PCR procedure to achieve a highly sensitive detection of B. mallei and B. pseudomallei.
Rhodococcus equi - ultrasensitive	B0026	Nasopharyngeal swab, or 1 ml tracheal wash, or 1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 1ml feces, shipped overnight at room temperature.	2 business days	Qualitative real time PCR	Normal range: Nondetected Using a real time PCR procedure, this assay achieves a highly sensitive detection of pathogenic strains of Rhodococcus equi.
Potomac horse fever (Neorickettsia risticii) - ultrasensitive	B0028	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, fecal swab or 1 ml feces, shipped overnight at room temperature.	2 business days	Qualitative real time PCR	Normal range: Nondetected. Using a real time PCR procedure, this assay achieves a highly sensitive detection of Neorickettsia risticii, formerly known as Ehrlichia risticii, the etiologic agent of PHF (also known as Equine monocytic ehrlichiosis)
Lawsonia intracellularis - ultrasensitive	B0035	1 ml feces or fresh or fixed ileum tissue shipped overnight at room temperature; or frozen ileum tissue.	2 business days	Qualitative real time PCR	Normal range: Nondetected.
Clostridium difficile	B0037	Rectal swab or 1 ml fecal specimen, shipped overnight at room temperature.	2 business days	Qualitative PCR	Normal range: Nondetected. This assay differentiates pathogenic and non-pathogenic strains of C. difficile by detecting the toxin producing genes.
Salmonella - ultrasensitive	B0040	Preferred specimens: rectal swab, 1 ml fecal specimen or bacterial culture. Less preferred specimen: 1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube. Ship specimens overnight at room temperature.	2 business days	Qualitative real time PCR	Normal range: Nondetected. This assay utilizes an ultrasensitive real time PCR protocol to detect but not differentiate most recognized serotypes of Salmonella bacteria.
Leptospira - ultrasensitive	B0051	0.5 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.5 ml urine, shipped overnight at room temperature.	2 business days	Qualitative real time PCR	Normal range: Nondetected. This assay utilizes a real time PCR procedure to achieve a highly sensitive detection but not differentiation of Leptospira serovars.
E. coli O157:H7 - ultrasensitive	B0059	Rectal swab, 1 ml fecal specimen or bacterial culture, shipped overnight at room temperature.	2 business days	Qualitative real time PCR	Normal range: Nondetected. This assay is specific for E. coli strain O157:H7 and does not detect other unrelated E. coli strains.
Clostridium species screen	B0061	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or rectal swab or 1 ml fecal specimen. Ship specimens overnight at room temperature.	2 business days	Qualitative real time PCR	Normal range: Nondetected. Assay detects but does not differentiate several common Clostridium species, including C. difficile, C. piliforme and C. perfringens. Assay DOES NOT detect Clostridium botulinum.
Yersinia pseudotuberculosis - ultrasensitive	B0062	Rectal swab or 1 ml fecal specimen, or 1 ml tissue, or 1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube. Ship specimens overnight at room temperature.	2 business days	Qualitative real time PCR	Normal range: Nondetected.
Helicobacter species screen - ultrasensitive	P0010	1ml gastric lavage or feces or tissue shipped overnight at room temperature; or tissue shipped frozen.	2 business days	Qualitative nested PCR	Normal range: Nondetected. This screen detects but does not differentiate Helicobacter species, including H. pylori, H. heilmannii, H. bilis, H. hepaticus and others, utilizing a nested PCR procedure to achieve highly sensitive detection. See assay P0011 for Helicobacter species identification.
Helicobacter species identification by ultrasensitive PCR and RFLP	P0011	1ml gastric lavage or feces or tissue shipped overnight at room temperature; or tissue shipped frozen.	2 business days	Qualitative nested PCR and restriction fragment length polymorphism	Normal range: Nondetected. Assay detects and differentiates Helicobacter species including H. pylori, H. heilmannii, H. bilis, H. hepaticus and others. This assay utilizes a nested PCR procedure to achieve a highly sensitive detection of Helicobacter bacteria, and an additional Restriction Fragment Length Polymorphism (RFLP) protocol to differentiate and identify Helicobacter species.
Equine respiratory panel	P0013	Tracheal wash or nasopharyngeal swab, shipped overnight at room temperature.	3 business days	Qualitative PCR, qualitative reverse transcription PCR and qualitative real time PCR	Normal range: Nondetected. Assay detects and differentiates equine arteritis virus, equine herpesvirus 1, equineherpesvirus 4, equine influenza A, Rhodococcus equi and Streptococcus equi subspecies equi and zooepidemicus.
Equine neurological panel	P0014	Preferred samples: 1 ml cerebrospinal fluid or brain tissue, shipped overnight at room temperature; or frozen CSF or tissue, shipped frozen. Less preferred sample: 1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, shipped overnight at room temperature.	3 business days	Qualitative PCR, qualitative real time PCR and qualitative reverse transcription real time PCR	Normal range: Nondetected. Assay detects and differentiates Eastern Equine Encephalitis, Western Equine Encephalitis, West Nile Virus, Equine herpes Virus type 1, Equine Protozoal Myeloencephalitis (includes Sarcocystis neurona, Neospora hughesi, Neospora caninum and Toxoplasma gondii)
Equine GI / diarrhea panel	P0015	Rectal swab, 1 ml fecal specimen or bacterial culture, shipped overnight at room temperature.	3 business days	Qualitative PCR and qualitative real time PCR	Normal range: Nondetected. Assay detects and differentiates Salmonella, Ehrlichia risticii, Clostridium difficile and Lawsonia intracellularis.
Enteric E. coli panel	P0016	Rectal swab, 1 ml fecal specimen or bacterial culture, shipped overnight at room temperature.	3 business days	Qualitative PCR	Normal range: Nondetected. Panel detects and differentiates these clinically important strains of enteric E. coli: ETEC, EHEC, EIEC, EPEC, EAEC.
Equine protozoal myeloencephalitis (EPM) panel	P0017	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 1 ml cerebrospinal fluid or brain tissue, shipped overnight at room temperature; or frozen CSF or tissue, shipped frozen.	3 business days	Qualitative PCR and qualitative real time PCR	Normal range: Nondetected. Assay detects and differentiates Sarcocystis neurona, Neospora hughesi, Neospora caninum and Toxoplasma gondii.
Equine breeding panel	P0024	Urethrogenital swab, cervical swab or 1 ml semen, shipped overnight at room temperature.	3 business days	Qualitative PCR, Qualitative reverse transcription PCR and qualitative real time PCR	Normal range: Nondetected. Panel detects and differentiates contagious equine metritis, equine arteritis virus, equine herpesvirus type I, Strep zoo and leptospirosis.
West Nile virus - ultrasensitive	S0048	Preferred samples: 1 ml CSF or tissue shipped overnight at room temperature, or 1 ml frozen tissue shipped frozen. Less preferred samples: 1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, serum or plasma, shipped overnight at room temperature; or frozen whole blood, serum or plasma, shipped frozen.	2 business days	Qualitative reverse transcription real time PCR	Normal range: Nondetected
Venezuelan equine encephalitis - ultrasensitive	S0054	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 1 ml CSF, serum, plasma or tissue, shipped overnight at room temperature; or 1 ml frozen serum, plasma, tissue or CSF, shipped frozen.	2 business days	Qualitative reverse transcription real time PCR	Normal range: Nondetected.
Eastern equine encephalitis - ultrasensitive	S0055	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 1 ml CSF, serum, plasma or tissue, shipped overnight at room temperature; or 1 ml frozen serum, plasma, tissue or CSF, shipped frozen.	2 business days	Qualitative reverse transcription real time PCR	Normal range: Nondetected.
Western equine encephalitis - ultrasensitive	S0056	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 1 ml CSF, serum, plasma or tissue, shipped overnight at room temperature; or 1 ml frozen serum, plasma, tissue or CSF, shipped frozen.	2 business days	Qualitative reverse transcription real time PCR	Normal range: Nondetected.
St. Louis encephalitis - ultrasensitive	S0057	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 1 ml CSF, serum, plasma or tissue, shipped overnight at room temperature; or 1 ml frozen serum, plasma, tissue or CSF, shipped frozen.	2 business days	Qualitative reverse transcription real time PCR	Normal range: Nondetected.
African Horse Sickness - ultrasensitive	S0061	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or plasma, serum or tissue, shipped overnight at room temperature; or 1 ml frozen plasma, serum or tissue, shipped frozen.	2 business days	Qualitative reverse transcription real time PCR	Normal range: Nondetected.
Japanese encephalitis - ultrasensitive	S0062	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 1 ml CSF, serum, plasma or tissue, shipped overnight at room temperature; or 1 ml frozen serum, plasma, tissue or CSF, shipped frozen.	2 business days	Qualitative reverse transcription real time PCR	Normal range: Nondetected.
Equine infectious anemia - ultrasensitive	S0064	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 1 ml tissue, shipped overnight at room temperature; or 1 ml frozen tissue, shipped frozen.	2 business days	Qualitative real time PCR	Normal range: Nondetected.
Equine arteritis virus - ultrasensitive	S0066	Nasopharyngeal swab, or 1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 1 ml semen, or 1 ml tissue, shipped overnight at room temperature; or 1 ml frozen semen or 1 ml frozen tissue, shipped frozen.	2 business days	Qualitative reverse transcription real time PCR	Normal range: Nondetected. This assay achieves a highly sensitive detection of most of the EAV strains reported in the literature.
Vesicular stomatitis - ultrasensitive	S0068	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or lesion swab, nasal wash, nasopharyngeal swab or tissue, shipped overnight at room temperature; or 1 ml tissue, shipped frozen.	2 business days	Qualitative reverse transcription real time PCR	Normal range: Nondetected. This assay achieves a highly sensitive detection and differentiation of the New Jersey and Indiana serotypes of the vesicular stomatitis virus.
Equine herpesvirus type I	S0071	Nasopharyngeal swab, or 1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or cerebrospinal fluid, or tissue, shipped overnight at room temperature; or tissue shipped frozen.	2 business days	Qualitative PCR	Normal range: Nondetected
Equine herpesvirus type II	S0072	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or nasopharyngeal swab, or bronchoalveolar lavage, or tissue, shipped overnight at room temperature; or tissue shipped frozen.	2 business days	Qualitative PCR	Normal range: Nondetected
Equine herpesvirus type III	S0073	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or lesion swab or tissue, shipped overnight at room temperature; or tissue shipped frozen.	2 business days	Qualitative PCR	Normal range: Nondetected
Equine herpesvirus type IV	S0074	Nasopharyngeal swab, or 1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or tissue, shipped overnight at room temperature; or tissue shipped frozen.	2 business days	Qualitative PCR	Normal range: Nondetected
Equine herpesvirus type V	S0075	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or nasopharyngeal swab, or tissue, shipped overnight at room temperature; or tissue shipped frozen.	2 business days	Qualitative PCR	Normal range: Nondetected
Influenza - ultrasensitive	S0077	Preferred sample: equine nasopharyngeal swab, shipped overnight at room temperature. Less preferred sample: 1 ml equine whole blood in EDTA (purple top) or ACD (yellow top) tube, shipped overnight at room temperature.	2 business days	Qualitative reverse transcription real time PCR	Normal range: Nondetected. Using a real time PCR procedure, this assay achieves a highly sensitive detection but not differentiation of influenza viruses, including strains H5N1, H5N2, H1N1, H2N2, H3N8, H4N6, H7N7, H8N4 and H9N2.
Rotavirus screen - ultrasensitive	S0130	0.5 ml feces, shipped overnight at room temperature.	2 business days	Qualitative reverse transcription real time PCR	Normal range: Nondetected. Assay detects but does not differentiate many common strains of rotavirus.
Toxoplasma gondii	X0002	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 1 ml amniotic fluid, CSF or tissue, shipped overnight at room temperature; or tissue shipped frozen.	2 business days	Qualitative PCR	Normal range: Nondetected
Sarcocystis neurona - ultrasensitive	X0004	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 1 ml cerebrospinal fluid or tissue, shipped overnight at room temperature; or frozen tissue shipped frozen.	2 business days	Qualitative real time PCR	Normal range: Nondetected
Babesia equi	X0005	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, shipped overnight at room temperature.	2 business days	Qualitative PCR	Normal range: Nondetected
Babesia caballi	X0006	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, shipped overnight at room temperature.	2 business days	Qualitative PCR	Normal range: Nondetected
Trypanosoma evansi (surra) - ultrasensitive	X0008	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or tissue, shipped overnight at room temperature; or frozen tissue shipped frozen.	2 business days	Qualitative real time PCR	Normal range: Nondetected
Neospora hughesi - ultrasensitive	X0009	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 1 ml plasma, serum or CSF, shipped overnight at room temperature; or 1 ml frozen plasma, serum or CSF.	2 business days	Qualitative real time PCR	Normal range: Nondetected.
Neospora caninum	X0011	1 ml feces, or 1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 1 ml aborted material, CSF, brain or heart tissue, shipped overnight at room temperature; or frozen tissue shipped frozen.	2 business days	Qualitative PCR	Normal range: Nondetected.
Trichomonas	X0013	Preferred specimens: rectal, vaginal, oral or other mucous secretion swab, or 1 ml fecal specimen, shipped overnight at room temperature. Less preferred specimens: 1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or fresh, fixed or paraffin-embedded tissue shipped overnight at room temperature, or frozen tissue shipped frozen.	2 business days	Qualitative PCR	Normal range: Nondetected.
Horse tapeworms (Anoplocephala perfoliata, Anoplocephala magna, and Anoplocephaloides mamillana) - ultrasensitive	X0015	1 ml feces	2 business days	Qualitative multiplex real time PCR	Normal range: Nondetected. This assay utilizes a multiplex real time PCR protocol to achieve a highly sensitive detection but not differentiation of the three most commonly-encountered tapeworm species in horses.
Strongyles worms - ultrasensitive	X0016	1 ml feces	2 business days	Qualitative real time PCR	Normal range: Nondetected. This assay utilizes a real time PCR protocol to achieve a highly sensitive detection but not differentiation of most strongyles species in horses.
Giardia - ultrasensitive	X0017	1 ml feces	2 business days	Qualitative real time PCR	Normal range: Nondetected.
Trypanosoma equiperdum - ultrasensitive	X0018	1 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or tissue, shipped overnight at room temperature; or frozen tissue shipped frozen.	2 business days	Qualitative real time PCR	Normal range: Nondetected

*For specimen types other than those listed, please call to confirm specimen acceptability and shipping instructions. For liquid, tissue, swab or fecal specimens, if there will be a delay in shipping or during very warm weather, refrigerate the specimen until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. Dry card samples can simply be mailed at room temperature. See shipping instructions for more information.

Our master menu lists all assays for all host species.