Strongyles worms in horses
Ultrasensitive qualitative detection but not differentiation
of most strongyles species in horses, by real time PCR.
the subfamily Cyathostominae (cyathostomins), order Strongylida,
are the most common parasites of horses. More than 50 species
within 13 genera have been documented, including
Cylicocyclus auriculatus, Cylicocyclus radiatus, Cylicocyclus
insigne, Cylicocyclus leptostomum, Cylicocyclus nassatus,
Cylicostephanus bidentatus, Cylicostephanus calicatus,
Cylicostephanus goldi, Cylicostephanus longibursatus,
Cylicostephanus minutus, Cyathostomum pateratum, Cyathostomum
catinatum, Coronocyclus coronatus, Coronocyclus labiatus
Tridentoinfundibulum gobi. Infected horses often
carry five to ten common species.
infected with these parasites may develop various syndromes
including larval cyathostominosis, a fatal enteritis that occurs
secondary to synchronized reactivation of inhibited larvae from
the intestinal mucosa. The mortality rate is 50% even when
intensive treatment is given. Other syndromes induced by these
nematodes include a protein-losing enteropathy with loss of
condition and peripheral edema, or mild weight loss, poor
appetite and lethargy with disordered intestinal motility. The
association between the individual strongyles species and the
development of these different clinical syndromes is not yet
have been exposed to these parasites do not always develop
immunity to the parasites. Infected horses can contaminate
pastures throughout their lives.
method of detecting strongyles infections is to remove the adult
worms at necropsy and examine their morphology, which is of
little value in clinical veterinary practice. Detecting eggs or
larvae in stool samples is difficult and time-consuming using
morphological parameters. However, molecular detection by PCR is
a highly sensitive and specific technique for detecting these
Help confirm the disease causing agent
Shorten the time required to confirm the presence of
Help ensure that herds are free of strongyles
Early prevention of spread of these parasites among a
herd or to other animals
Minimize human exposure to strongyles
Specimen requirements: 0.2 ml feces or rectal swab.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
Qualitative real time PCR