Respiratory symptoms got you breathless? Try our equine respiratory PCR panel -- we test for 7 respiratory bacteria and viruses from 1 swab.

Neurological symptoms got you down? Try our equine neurological PCR panel -- we test for 5 neurological diseases from 1 CSF or tissue sample.

Diarrhea got you on the run? Try our equine GI / diarrhea PCR panel -- we test for 4 GI diseases from 1 fecal or swab sample.

Oh baby! Our equine breeding/abortion PCR panel tests for 5 diseases affecting breeding success from 1 swab or semen sample.

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For our international clients: Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere. Samples are small, light and stable at room temperature.

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Zoologix performs equine PCR tests for...

African horse sickness

Anaplasma phagocytophilum

Anoplocephala

Anoplocephaloides

Aspergillus

Babesia

Borna virus

Borrelia burgdorferi

Burkholderia mallei and pseudomallei

Clostridium difficile

Clostridium species

Contagious equine metritis (CEM)

Coronaviruses

Dengue

Dourine

Eastern equine encephalitis (EEE)

E. coli O157:H7

E. coli panel

Equine adenoviruses

Equine arteritis virus (EAV)

Equine hepatitis virus

Equine herpesvirus
type 1

Equine herpesvirus
type 2

Equine herpesvirus
type 3

Equine herpesvirus
type 4

Equine herpesvirus
type 5

Equine infectious anemia (EIA)

Equine parvovirus

Equine piroplasmosis

Equine protozoal myeloencephalitis (EPM)

Equine rhinitis virus A

Equine rhinitis virus B

Giardia

Glanders

Helicobacter

Histoplasma

Horsepox virus

Influenza type A

Japanese encephalitis

Lawsonia intracellularis

Leptospirosis

Lyme disease

Melioidosis

Neospora caninum

Neospora hughesi

Piroplasmosis

Potomac horse fever

Rabies

Rhodococcus equi

Rotavirus

Sarcocystis neurona

St. Louis encephalitis

Strangles (Strep equi)

Streptococcus pneumoniae

Strongyles

Surra

Tapeworms

Taylorella equigenitalis

Theileria equi

Toxoplasma gondii

Trichomonas/
Tritrichomonas

Trypanosoma equiperdum

Trypanosoma evansi

Venezuelan equine encephalitis (VEE)

Vesicular stomatitis

West Nile virus (WNV)

Western Equine Encephalitis (WEE)

Yersinia enterocolitica

Yersinia pseudotuberculosis

Genetic tests for...

Hyperkalemic periodic paralysis


Rabies PCR test for horses
equine assay data sheet

Rabies NOTE: THIS TEST IS NOT PERFORMED ON SAMPLES TAKEN FROM LIVESTOCK OWNED OR LOCATED IN THE STATE OF CALIFORNIA.

Test code: S0116 - Ultrasensitive qualitative detection of rabies virus by reverse transcription coupled real time polymerase chain reaction.

Rabies virus, a nonsegmented, negative-stranded RNA virus, is a member of the Rhabdoviridae family. This family includes at least three genera of animal viruses, Lyssavirus, Ephemerovirus, and Vesiculovirus. The genus Lyssavirus includes rabies virus, Lagos bat virus, Mokola virus, Duvenhage virus, European bat virus 1 & 2 and Australian bat virus.

Rabies virus can cause fatal acute encephalitis in all mammalian hosts, including humans, dogs, cats, ferrets, pigs, livestock and many other species. However, only a few species are important as reservoirs for the disease. In the United States, several distinct rabies virus variants have been identified in raccoons, skunks, foxes, coyotes and several species of insectivorous bats.

Transmission of rabies virus usually begins when an uninfected animal contacts the saliva of an infected host animal. Various routes of transmission have been documented, including contamination of mucous membranes (ie eyes, nose, and mouth) and even aerosol transmission. However, the most common mode of rabies virus transmission is a bite from an infected host animal.

Initial symptoms of rabies infection in animals include lethargy, fever, vomiting, and anorexia. Signs progress within days to cerebral dysfunction, ataxia, weakness, paralysis, seizures, difficulty breathing, difficulty swallowing, excessive salivation, abnormal behavior, aggression, and/or self-mutilation.

Serology testing has been used to diagnose rabies virus exposure in animals. Direct fluorescent antibody testing is most frequently used to diagnose rabies. This test requires brain tissue from the animal suspected of being rabid. The test can only be performed post-mortem and is not suitable for testing live animals that may have contacted the virus. However, since animals may have had prior exposure to the virus, serology testing may not be specific in confirming the current presence of the virus. An extensive and time-consuming serology titering study may be required to prove the animal’s recent exposure.

Molecular detection by PCR is a rapid, sensitive and specific method to identify the presence of the rabies virus in a sample. The PCR test can be performed on saliva, spinal fluid or a bite lesion swab or biopsy.

See the US Centers for Disease Control (CDC) rabies website at http://www.cdc.gov/rabies/ for additional information on the diagnosis of rabies.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of rotavirus
  • Help ensure that equine facilities are free of rotaviruses
  • Early prevention of spread of rotaviruses among a facility
  • Minimize human exposure to rotaviruses
  • Safety monitoring of biological products that derive from susceptible animals

References:
http://www.cdc.gov/rabies/ (Centers for Disease Control and Prevention’s rabies information website)     

Specimen requirements:  Buccal swab, or 0.2 ml CSF, or 0.2 ml fresh or frozen brain stem tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time polymerase chain reaction

Normal range: Nondetected

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