equine assay data sheet
Equine herpesvirus type II (EHV-2), formerly "equine
detection of equine herpesvirus type II by real time polymerase chain
herpesvirus type II (EHV-2) is a DNA virus belonging to the
gammaherpesvirus family. This virus infects B cells and is
related to other lymphotropic herpesviruses such as Epstein-Barr
virus (EBV, which causes glandular fever) and human herpesvirus
type 8 (HHV-8), also known as Kaposi's sarcoma herpesvirus.
Equine herpesvirus II was previously known as "equine CMV
highly prevalent (approaching 100%) in horse populations (Borchers
et al., 1999) and has been proposed to cause, or to be a
predisposing factor for, a number of distinct syndromes. These
include “loss of performance”, conjunctivitis (Kershaw et al.,
2001) and respiratory tract disease. EHV-2 has also been
suggested as a possible transactivating factor triggering or
exacerbating EHV-1 or EHV-4 reactivation and disease. While the
association of EHV-2 with particular diseases is subject to
debate, it is clear that this virus has the potential to escape
and subvert host immune responses.
detection of EHV-2 is of limited use to determine the
re-activation of this herpes virus. EHV-2 can remain latent in
affected animals while shedding at levels adequate to infect
other horses. Culture identification of EHV-2 is slow and has
low sensitivity. Molecular detection of EHV-2 by PCR is the most
sensitive, specific and accurate tool in assessing the
infectivity of an affected horse.
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of EHV-2 infection
Help ensure that horse populations are free of EHV-2
Early prevention of spread of this virus
Minimize personnel exposure to this virus
Safety monitoring of biological products that derive
Slater, J. D., Borchers, K., Thackray, A. M. and Field, H. J.
(1994) The trigeminal ganglion is a location for equine
herpesvirus 1 latency and reactivation in the horse. J. Gen.
Virol. 75: 2007–2016.
Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top)
tube, or nasopharyngeal swab, or 0.2 ml broncheoalveolar lavage,
or 0.2 ml fresh, frozen or fixed tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
Qualitative real time PCR