Respiratory symptoms got you breathless? Try our equine respiratory PCR panel -- we test for 7 respiratory bacteria and viruses from 1 swab.

Neurological symptoms got you down? Try our equine neurological PCR panel -- we test for 5 neurological diseases from 1 CSF or tissue sample.

Diarrhea got you on the run? Try our equine GI / diarrhea PCR panel -- we test for 4 GI diseases from 1 fecal or swab sample.

Oh baby! Our equine breeding/abortion PCR panel tests for 5 diseases affecting breeding success from 1 swab or semen sample.

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For our international clients: Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere. Samples are small, light and stable at room temperature.

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Zoologix performs equine PCR tests for...

African horse sickness

Anaplasma phagocytophilum

Aspergillus

Babesia

Borna virus

Borrelia burgdorferi

Burkholderia mallei and pseudomallei

Clostridium difficile

Clostridium species

Contagious equine metritis (CEM)

Coronaviruses

Dourine

Eastern equine encephalitis (EEE)

E. coli O157:H7

E. coli panel

Equine adenoviruses

Equine arteritis virus (EAV)

Equine herpesvirus
type 1

Equine herpesvirus
type 2

Equine herpesvirus
type 3

Equine herpesvirus
type 4

Equine herpesvirus
type 5

Equine infectious anemia (EIA)

Equine piroplasmosis

Equine protozoal myeloencephalitis (EPM)

Giardia

Glanders

Helicobacter

Histoplasma

Horsepox virus

Influenza

Japanese encephalitis

Lawsonia intracellularis

Leptospirosis

Lyme disease

Melioidosis

Neospora caninum

Neospora hughesi

Piroplasmosis

Potomac horse fever

Rabies

Rhodococcus equi

Rotavirus

Sarcocystis neurona

St. Louis encephalitis

Strangles (Strep equi)

Streptococcus pneumoniae

Strongyles

Surra

Tapeworms

Taylorella equigenitalis

Theileria equi

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma equiperdum

Trypanosoma evansi

Venezuelan equine encephalitis (VEE)

Vesicular stomatitis

West Nile virus (WNV)

Western Equine Encephalitis (WEE)

Yersinia enterocolitica

Yersinia pseudotuberculosis

Genetic tests for...

Hyperkalemic periodic paralysis


Borna virus PCR test

equine assay data sheet

Borna virus

Test code:
S0207 - Ultrasensitive qualitative detection of bornavirus by reverse transcription coupled real time PCR.

Borna disease virus (BDV) is an enveloped nonsegmented negative-strand RNA virus with a genome size of about 9 kb. The virus is of member of the Mononegavirales order. The Mononegavirales also include Filoviridae (eg Marburg and Ebola viruses), Paramyxoviridae (eg mumps, measles virus), and Rhabdoviridae (eg rabies, vesicular stomatitis virus).

This viral disease was first described more than 200 years ago in a small town named Borna in Saxony in southern Germany. It is a fatal neurologic disease of horses and sheep. A large number of horses died during an epidemic in 1885. Although outbreaks of Borna disease are rare, serological survey has indicated that many horses in various geographic regions have been exposed to the virus. This suggests that natural infection of horses with this virus may be subclinical.

Although horses are the natural host of the virus, other equidae, sheep, cattle, rabbits, goats, deer, alpacas, llamas, cats, pygmy hippopotamus, sloths and ostriches can be infected with BDV. The virus is transmitted by direct contact with saliva, nasal discharge or conjunctival secretions of infected animals.  Direct exposure to contaminated food or water can also be a source of infection.

Infected horses or sheep usually take about 4 weeks to show signs of infection, but the signs are non-specific. These signs include hyperthermia, anorexia, colic, and constipation in the initial phase of the disease. During the acute phase, neurologic signs such as ataxia, depression, circular movement, standing in awkward positions, collapsing, running into obstacles, and paralysis, may develop. Clinical symptoms last 1 to 3 weeks, and death rates for diseased horses are 80% to 100%.

Diagnosis of Borna disease can be by serological methods or by molecular methods such as polymerase chain reaction. PCR is rapid, sensitive and specific (Wensman et al., 2012), and does not require infected animals to develop full immune responses. Thus, PCR is especially suitable for early detection of the virus.

Utilities:

  • Help confirm the disease causing agent
  • Identify Borna virus carriers
  • Help ensure that herds are free of Borna virus
  • Early prevention of spread of this virus among horses and other animals
  • Minimize human exposure to this virus
  • Safety monitoring of biological products that derive from animals

References:
Wensman, J.J., Jäderlund, K.H., Gustavsson, M.H., Hansson-Hamlin, H., Karlstam, E., Lilliehöök, I., Oström, I.L., Belák, S., Berg, M. and Holst, B.S. (2012) Markers of Borna disease virus infection in cats with staggering disease. J. Feline Med. Surg. 14:573-582.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or oral swabs, or nasal swabs, or 0.2 ml fresh or frozen tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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