Respiratory symptoms got you breathless? Try our equine respiratory PCR panel -- we test for 7 respiratory bacteria and viruses from 1 swab.

Neurological symptoms got you down? Try our equine neurological PCR panel -- we test for 5 neurological diseases from 1 CSF or tissue sample.

Diarrhea got you on the run? Try our equine GI / diarrhea PCR panel -- we test for 4 GI diseases from 1 fecal or swab sample.

Oh baby! Our equine breeding/abortion PCR panel tests for 5 diseases affecting breeding success from 1 swab or semen sample.

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For our international clients: Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere. Samples are small, light and stable at room temperature.

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Zoologix performs equine PCR tests for...

African horse sickness

Anaplasma phagocytophilum

Anoplocephala

Anoplocephaloides

Aspergillus

Babesia

Borna virus

Borrelia burgdorferi

Burkholderia mallei and pseudomallei

Clostridium difficile

Clostridium species

Contagious equine metritis (CEM)

Coronaviruses

Dengue

Dourine

Eastern equine encephalitis (EEE)

E. coli O157:H7

E. coli panel

Equine adenoviruses

Equine arteritis virus (EAV)

Equine hepatitis virus

Equine herpesvirus
type 1

Equine herpesvirus
type 2

Equine herpesvirus
type 3

Equine herpesvirus
type 4

Equine herpesvirus
type 5

Equine infectious anemia (EIA)

Equine parvovirus

Equine piroplasmosis

Equine protozoal myeloencephalitis (EPM)

Equine rhinitis virus A

Equine rhinitis virus B

Giardia

Glanders

Helicobacter

Histoplasma

Horsepox virus

Influenza type A

Japanese encephalitis

Lawsonia intracellularis

Leptospirosis

Lyme disease

Melioidosis

Neospora caninum

Neospora hughesi

Piroplasmosis

Potomac horse fever

Rabies

Rhodococcus equi

Rotavirus

Sarcocystis neurona

St. Louis encephalitis

Strangles (Strep equi)

Streptococcus pneumoniae

Strongyles

Surra

Tapeworms

Taylorella equigenitalis

Theileria equi

Toxoplasma gondii

Trichomonas/
Tritrichomonas

Trypanosoma equiperdum

Trypanosoma evansi

Venezuelan equine encephalitis (VEE)

Vesicular stomatitis

West Nile virus (WNV)

Western Equine Encephalitis (WEE)

Yersinia enterocolitica

Yersinia pseudotuberculosis

Genetic tests for...

Hyperkalemic periodic paralysis


Equine herpes 5 PCR test
equine assay data sheet

Equine herpesvirus type V (EHV-5)

Test code:
S0075 - Ultrasensitive qualitative detection of equine herpesvirus type V by real time polymerase chain reaction

 

Equine herpesvirus type 2 (EHV-2) and type 5 (EHV-5) were recently re-classified as members of the gamma herpesviridiae family (Browning and Studder, 1987; Telford et al., 1993). Unlike EHV-2, which is widely spread in horse populations worldwide, EHV-5 has so far only been isolated in a few countries, including Switzerland, Germany, Australia and New Zealand. In Australia, EHV-5 was originally discovered in horses suffering from upper respiratory disease (Browning and Studdert, 1987). A difference in the prevalence of EHV-2 and EHV-5 was also observed in Australia where 31 % of the tested horses were positive to EHV-2 while only 16 % were positive to EHV-5.

Studies have shown that EHV-5 infection is very common in young horses and EHV-5 infections persist in young horses for at least several months. However, EHV-5 is not specifically associated with respiratory disease. In contrast, EHV-2 is less commonly isolated from young horses and its detection is associated with the occurrence of mild respiratory disease.

Serological separation of EHV-2 and EHV-5 is very difficult because of the high degree of similarity in their protein sequences. Reliable differentiation of EHV-5 and EHV-2 relies on molecular detection methods. PCR is the most sensitive and specific technique for detection of each of these viruses.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of EHV-5 infection
  • Help ensure that horse populations are free of EHV-5
  • Early prevention of spread of this virus
  • Minimize personnel exposure to this virus
  • Safety monitoring of biological products that derive from horses

References:
Browning, G.F. and Studdert, M.J. (1987) Genomic heterogeneity of equine betaherpesviruses. J. Gen. Virol. 68: 1441-1447.
Telford, E.A.R., Studdert, M.J., Agius, C.D., Watson, M.S., Aird, H.C. and Davison, A.J. (1993) Equine herpesviruses 2 and 5 are gamma-herpesviruses. Virology 195: 492-499.

Specimen requirements: Nasopharyngeal swab, or 0.2 ml whole blood in EDTA (purple top) tube, or 0.2 ml fresh, frozen or fixed tissue, or cellular suspensions (platelets, PBMCs, etc).

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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