equine assay data sheet
Glanders and Melioidosis
B0025 - Ultrasensitive qualitative detection of
pseudomallei by real time polymerase chain reaction.
This test detects but does not differentiate these two bacteria.
and Burkholderia pseudomallei cause glanders and melioidosis.
Melioidosis is endemic in Southeast Asia and northern Australia.
Septicemic melioidosis is a major cause of high morbidity and
mortality in Northeastern Thailand. Sporadic reports of
melioidosis in humans and animals occur throughout the world.
the other hand, is a serious infectious equine disease. Human
glanders is rare and is found primarily in veterinarians, horse
and donkey caretakers, abattoir workers (Eitzen et al., 1999)
and laboratory workers (Jenning, 1963). Glanders in humans is
acquired from infected animals or by ingestion or inhalation of
Burkholderia bacteria. Laboratory workers handling Burkholderia
may become infected by inhaling aerosols containing these
bacteria. The spectrum of disease ranges from asymptomatic
infection to fulminate septicemia, which needs rapid detection
and differentiation for specific treatment.
and B. pseudomallei species are very similar in their nutritional
and biochemical properties. Sequence analysis of the two
bacteria indicates DNA similarity of more than 80% in these two
species. For some sequences, such as 16S rRNA, homology of these
two bacteria is up to 100%.
Not only are
these species indistinguishable morphologically, it is also
difficult to distinguish them serologically. They produce
diseases in experimental animals that are practically identical,
both clinically and pathologically.
microbiological laboratories it is safer to examine highly
pathogenic micro-organisms under kill-conditions, so live
culturing of bacteria such as Burholderia should be avoided if
possible. PCR detection of these bacteria is useful because it
is rapid, sensitive, specific and safer than culture-based
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of B. mallei
and B. pseudomallei
Help ensure that horse populations are free of
B. mallei and
Early prevention of spread of these bacteria
Minimize personnel exposure to these bacteria
Safety monitoring of biological products that derive
Eitzen, E., Culpepper, R., Cieslak, T., Christopher, G., Rowe,
J. and Pavin, J. (1999) Editors, Glanders: medical management of
biological casualties handbook, US Army Medical Research
Institute Diseases Fort Detrick, Maryland.
(1963) Glanders. In: C. Charles, Editor, Diseases transmitted
from animals to man, Thomas Publisher, Springfield, pp. 262–264.
Detection of Burkholderia mallei and Burkholderia pseudomallei
Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top)
tube, or 0.2 ml sputum, pus or bacterial subculture.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
real time PCR