Mycoplasma haemofelis PCR test
dog and cat assay data sheet
Mycoplasma haemofelis (formerly
- aka "feline infectious anemia" or "feline hemotropic mycoplasmosis"
B0047 - Ultrasensitive qualitative detection of
Mycoplasma haemofelis by
real time polymerase chain reaction
B0047 is included
on P0021 - feline bloodborne panel
and on P0040 - feline anemia panel
in cats is caused by Mycoplasma
haemofelis, previously known as
Cats infected with this organism typically develop regenerative anemia
as the bacteria attack the cat's red blood cells.
Mycoplasma haemocanis, the
bacteria can be transmitted from one animal to another by
blood-feeding arthropods such as fleas and ticks.
M. haemofelis can also be
transmitted from female cats with clinical disease to their newborn
offspring in the absence of arthropod vectors. The severity of disease
produced by M. haemofelis
varies, from cats that are mildly anemic and without clinical
signs to cats that are markedly depressed and die as a result of
severe anemia. The most common clinical signs in ill cats are
depression, weakness, anorexia, weight loss and pale mucous membranes.
Studies have estimated the prevalence of this bacterium in the feline
population from 0.9 to 28%.
has not been successfully grown in agar or cell cultures due to its
fastidious growth requirements. Traditionally, diagnosis relied on
microscopic identification of organisms attached to the surfaces of
erythrocytes in Wright-Giemsa-stained peripheral blood smears.
However, this method not only lacks sensitivity but also is not
adequately specific because the morphology of
M. haemofelis can easily
be confused with other microorganisms such as rickettsia. Molecular
detection by PCR is the best way to detect
M. haemofelis in blood of
infected cats. Using PCR analysis, it is possible to detect the
pathogen in blood samples obtained from cats during parasitemia,
during most of the carrier phase, and even after challenge with
immunosuppressive drugs which would prevent detection by serological
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of Mycoplasma
Help ensure that cat populations are free of
Early prevention of spread of
M. haemofelis among a
group of cats
Minimize human exposure to
requirement: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen
types, if there will be a delay in shipping, or during very warm
weather, refrigerate specimens until shipped and ship with a cold pack
unless more stringent shipping requirements are specified. Frozen
specimens should be shipped so as to remain frozen in transit. See
shipping instructions for more
2 business days
Qualitative real time