Feline leukemia PCR test
dog and cat assay data sheet
leukemia virus (FeLV)
S0111 - Ultrasensitive qualitative detection of feline leukemia
virus by real time polymerase chain reaction. This assay detects
proviral DNA of feline leukemia virus in sample types containing cellular material,
but does not differentiate types A, B and C.
S0132 - Ultrasensitive qualitative detection of feline leukemia
virus RNA by reverse transcription coupled real time polymerase chain reaction. This assay detects viral RNA of feline leukemia virus in
serum, plasma or blood, but does not differentiate types A, B and C.
S0171 - Ultrasensitive quantitative detection of feline leukemia
virus proviral DNA by real time polymerase chain reaction. This assay
feline leukemia virus in sample types containing cellular material, but does not differentiate types A, B and C.
S0172 - Ultrasensitive quantitative detection of feline leukemia
virus RNA by reverse transcription real time
polymerase chain reaction. This assay quantitates viral RNA of feline leukemia virus in serum, plasma or blood, but does not differentiate types A, B and C.
S0111 is included
onP0021 - feline bloodborne panel
and on P0040 - feline anemia
virus (FeLV) infection is one of the leading causes of death in cats.
In the United States, 2% to 3% of all cats are infected with FeLV.
FeLV is a
retrovirus which infects domestic cats and some wild felines. There
are three types: FeLV-A, FeLV-B and FeLV-C. Cats can be infected with
one, two or all three types simultaneously. Infection with FeLV-A can
result in severe immunosuppression. When infected with FeLV-B, cats
develop neoplastic disease (ie tumors and other abnormal tissue
growths) more readily than cats infected only with FeLV-A. FeLV-C is
seen in only about 1% of FeLV-infected cats and causes severe anemia.
infection, the virus replicates in the tonsils and pharyngeal lymph
nodes. It spreads via the bloodstream to other parts of the body,
especially other lymph nodes, bone marrow and intestinal tissue, where
it continues to replicate. Viremia, the presence of virus in the
blood, usually begins 2 to 4 weeks after the initial infection.
There are several
risk factors for FeLV infection. Ill cats are four times more likely
than healthy cats to become infected with FeLV. Researchers estimate
that about 50% of cats with severe bacterial infections and 75% of
cats with toxoplasmosis also have FeLV infections. Outdoor cats are
more likely than indoor cats to be infected with FeLV. Less than 1% of
healthy indoor cats in the United States are infected with FeLV,
compared to 1% to 2% of healthy outdoor cats, and more than 13% of ill
FeLV can easily be
transmitted via saliva. It can also spread through infected urine,
tears and feces, and to kittens during gestation and nursing. Twenty
percent of FeLV-positive mothers pass the virus to their kittens.
Other modes of transmission include bites from infected cats, blood
transfusions, mutual grooming, nose-to-nose contact, shared food
dishes and water bowls, shared litter trays and sneezing.
does not provide absolute protection against FeLV infection, so
chronically ill cats should be tested for FeLV even if vaccinated.
diagnosis of FeLV has been used to diagnose FeLV-infected cats.
However, cats with weakened immune response due to other infections or
diseases will not be reliably detected by serological methods.
Molecular detection by PCR overcomes this problem, because PCR detects
the presence of the pathogen itself rather than the host animal’s
immune response. PCR is rapid, highly sensitive and specific. A recent
study (Gomes-Keller et al., 2006) has shown that FeLV proviral DNA
detection in blood by PCR provides significantly higher sensitivity,
specificity and positive and negative predictive values than serology.
Gomes-Keller, M.A., Gönczi, E., Tandon, R., Riondato, F.,
Hofmann-Lehmann, R.,Meli, M.L. and Lutz, H. (2006) Detection of feline
leukemia virus RNA in saliva from naturally infected cats and
correlation of PCR results with those of current diagnostic methods.
J. Clin. Microbiol. 44:916-22.
0.2 ml whole
blood in EDTA (purple top) tube, or other sample types containing cellular material.
S0132: 0.2 ml whole blood in EDTA (purple top) tube,
or 0.2 ml serum or plasma.
ml whole blood in EDTA (purple top) tube, or other
sample types containing cellular material.
S0172: 0.2 ml whole blood in EDTA (purple top) tube,
or 0.2 ml serum or plasma.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen
types, if there will be a delay in shipping, or during very warm
weather, refrigerate specimens until shipped and ship with a cold pack
unless more stringent shipping requirements are specified. Frozen
specimens should be shipped so as to remain frozen in transit. See
shipping instructions for more
2 business days
Qualitative real time PCR targeting FeLV cellular proviral DNA
Qualitative reverse transcription real time PCR targeting FeLV RNA
Quantitative real time PCR targeting FeLV cellular proviral DNA
Quantitative reverse transcription real time PCR targeting FeLV RNA