Mycoplasma cynos PCR test
dog and cat assay data sheet
Mycoplasma cynos
Test code:
B0112 - Ultrasensitive qualitative detection of
Mycoplasma cynos by real
time polymerase chain reaction
Mycoplasmas belong to the class Mollicutes and contain the smallest
known genomes of any independently viable bacterial species. They have
no cell wall. They are highly fastidious, difficult to culture, and
slow growing. Mycoplasma
infections are highly contagious.
Mycoplasma species can
survive for weeks to months outside the host, and the environment can
therefore be a source of infection.
Among the different species of Mycoplasma bacteria,
Mycoplasma cynos has been identified as one of the causative
agents of canine infectious respiratory disease (CIRD) or “kennel
cough.” The ability of M. cynos
to initiate primary respiratory infection is not fully understood, but
this bacterium is a frequent co-infection in dogs with respiratory
infections initiated by other pathogens. Lung tissue damage caused by
viral infections promotes colonization by
Mycoplasma. Destruction and
loss of cilia and alveolar infiltration with macrophages and
neutrophils have been reproduced during experimental infection with
M. cynos. Mycoplasma
co-infections are linked to disease severity and duration,
particularly if it colonizes the lower respiratory tract; however,
M. cynos is sometimes
identified in the upper respiratory tract of dogs without clinical
disease. Younger dogs (< 1 year old) appear to me more likely to have
M. cynos infections than
older dogs.
Due to the fastidious growth requirements of
M. cynos, culture detection
is not effective. Molecular detection by PCR is recommended, as this
method is highly specific, sensitive and rapid.
Utilities:
-
Help confirm the disease causing agent
-
Shorten the time required to confirm a clinical
diagnosis of Mycoplasma cynos
infection
-
Help ensure that dog populations are free of
M. cynos
-
Early prevention of spread of
M. cynos among a group
of dogs
-
Minimize human exposure to
M. cynos
Specimen
requirement:
Nasal swab, or environmental surface swab, or 0.2 ml fresh, frozen or
fixed tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen
types, if there will be a delay in shipping, or during very warm
weather, refrigerate specimens until shipped and ship with a cold pack
unless more stringent shipping requirements are specified. Frozen
specimens should be shipped so as to remain frozen in transit. See
shipping instructions for more
information.
Turnaround
time:
2 business days
Methodology:
Qualitative real time
PCR
Normal range:
Nondetected
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