Feline sarcomavirus PCR test
dog and cat assay data sheet
sarcoma virus (FeSV)
S0190 - Ultrasensitive qualitative detection of feline sarcomavirus by
real time polymerase chain reaction
sarcoma viruses (FeSV)
arise as a result of recombination of feline leukemia virus (FeLV)
with parts of the host cell genome. The recombinant viruses have
deletions rendering them unable to replicate without the help of
wild-type FeLV. However, because these recombinant viruses still
contain a mutated cellular oncogene as a result of the recombination,
they are capable of causing tumors, mainly of fibroblastic cells.
FeSV has been reported
worldwide, but it is uncommon. FeSV-induced tumors are seen only in
cats persistently infected with FeLV. Since the tumors usually contain
both FeLV and FeSV, the tumors are transmissible experimentally, but
there is no evidence that natural cat-to-cat transmission occurs. In
fact, FeSV is found almost entirely in the tumor itself, and only
wild-type FeLV is shed from affected cats.
usually appear as multiple, ulcerative or nodular, non-healing skin
lesions, which recur after surgical excision. Metastasis to internal
organs may occur late in the course of the disease. All affected cats
have FeLV viremia. Unlike spontaneous fibrosarcomas of cats, which are
usually solitary and seen in older cats, most FeSV-induced tumors are
seen in cats 1-7 years old.
Diagnosis is usually
based on history, FeLV status and histological confirmation of
fibrosarcoma, although complete confirmation of the diagnosis requires
extensive laboratory studies, performed by PCR or other methods (Deim
et al., 2008).
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
Help ensure that feline populations are free of FeSV
Minimize human exposure to this virus
requirement: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or
0.2 ml fresh, frozen or fixed tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen
types, if there will be a delay in shipping, or during very warm
weather, refrigerate specimens until shipped and ship with a cold pack
unless more stringent shipping requirements are specified. Frozen
specimens should be shipped so as to remain frozen in transit. See
shipping instructions for more
2 business days
Qualitative real time PCR