Lawsonia PCR test for dogs and cats
dog and cat assay data sheet
B0035 - Ultrasensitive qualitative detection of
by real time polymerase chain reaction.
enteropathy, also known as proliferative ileitis, is caused by
infection with Lawsonia
intracellularis, an obligate intracellular, curve-shaped,
argyrophilic bacterium. The disease has been detected in domestic and
laboratory animals including primates, pig, horse, dog, rat, ferret,
guinea pig, rabbit and hamster. The disease has been reported
sporadically in foals 3-7 months of age and one outbreak involving 3
different breeding farms has been described. All reported cases were
in the eastern half of Canada or the United States. The swine industry
suffers the most significant impact from this disease. The disease has
two clinical manifestations in pigs: an acute hemorrhagic form often
called porcine hemorrhagic enteropathy, and a more chronic
proliferative form often referred as porcine intestinal adenomatosis.
contamination with feces of infected animals appears to be the most
important route of transmission of disease, but it is currently
unknown how long Lawsonia
intracellularis can remain infectious outside the animal.
Infection occurs most often during the post-weaning period, when
passive maternal immunity declines. After ingestion, the bacteria
infect intestinal proliferating crypt epithelial cells and multiply
within the apical cytoplasm. There is no evidence of infection of
tissues other than intestine. Most infected animals have subclinical
infection but shed the bacteria in their feces, leading to
environmental contamination. Clinical manifestation of an infection
can be triggered by stressors, such as overcrowding, transport, change
in diet, and experimental manipulation.
and isolation of the bacteria are difficult because cultured
enterocytes are required to support the growth of
Electron microscopy can be used to detect the curved bacterial rods in
apical cytoplasm of enterocytes but this is a very time-consuming
process and few laboratories have such capability. Immunohistochemical
detection can be performed on formalin-fixed, paraffin-embedded tissue
from affected intestine to detect the bacteria in following biopsy or
necropsy, but this is also a very time-consuming process. Molecular
detection using PCR is the most sensitive, rapid and specific method
to confirm presence of the
Lawsonia intracellularis genome within tissue samples
(Jones et al., 1993).
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of Lawsonia
Help ensure that animal populations are free of
Early prevention of spread of this bacterium
Minimize personnel exposure to this bacterium
Safety monitoring of biological products that derive
from susceptible animals
Jones, G. F., Ward, G.E., Murtaugh, M.P., Lin, G. and Gebhart, C.J.
(1993) Enhanced detection of intracellular organism of swine
proliferative enteritis, ileal symbiont intracellularis in feces by
polymerase chain reaction. J. Clin. Microbiol. 31:2611-2615.
requirements: 0.2 ml feces, or rectal swab, or 0.2 ml fresh, frozen or fixed ileum
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen
types, if there will be a delay in shipping, or during very warm
weather, refrigerate specimens until shipped and ship with a cold pack
unless more stringent shipping requirements are specified. Frozen
specimens should be shipped so as to remain frozen in transit. See
shipping instructions for more
2 business days
Qualitative real time