Ehrlichia PCR tests for dogs and cats
dog and cat assay data sheet
- Ultrasensitive qualitative detection of
Ehrlichia canis by real
time polymerase chain reaction. This assay
does not detect other
- Ultrasensitive screen for
by real time polymerase chain reaction. This assay
detects but does not differentiate
most common Ehrlichia
NOTE: TEST B0057 IS NOT PERFORMED ON
SAMPLES TAKEN FROM RUMINANTS OWNED OR LOCATED IN THE STATE OF CALIFORNIA.
B0057 is included
on P0025 - tickborne disease panel
and on P0039 canine anemia panel
ehrlichiosis is a disease of dogs, wolves and other canids. Occurring
worldwide, canine ehrlichiosis is also known by other names such as
'tracker dog disease,' 'tropical canine pancytopenia,' 'canine
hemorrhagic fever' and 'canine typhus.' The disease is caused by
ehrlichieae, tick-transmitted organisms that infect the leukocytes of
specific mammalian hosts. There are several ehrlichieae that can
infect dogs but Ehrlichia canis is the most common and severe one.
Disease caused by
E. canis typically
occurs in three phases. The initial acute phase is characterized by
fever, malaise, lymphadenomegaly, splenomegaly, thrombocytopenia,
leukopenia, and nonregenerative anemia. Symptoms subside in 2 to 4
weeks but are followed by a subclinical phase that persists for 2–3
months to years, during which infected dogs are carriers. Some dogs
subsequently enter a chronic phase, a period when severe clinical
ehrlichiosis occurs. E. canis
causes ocular disease and meningitis during this phase.
transmitted by the Brown Dog tick,
The immature form of the tick feeds on an animal infected with
Ehrlichia. When the tick
later feeds on another animal, the
Ehrlichia is passed on.
The disease can occur wherever Brown Dog ticks are found. Almost every
state in the United States has reported ehrlichiosis.
Dogs exposed to
E. canis infection
will develop an antibody response detectable by ELISA, but this immune
response does not prevent re-infection. ELISA does not distinguish
dogs with prior exposure from those with current infections, and it
cannot determine reinfection or current carrier status. Blood smear
examination can detect E. canis, but the sensitivity of this technique is very low.
Molecular detection by PCR, which is very specific and sensitive, is
useful to quickly identify this pathogen and confirm animals’
infection or carrier status.
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of Ehrlichia canis
Help ensure that dog populations are free of
Early prevention of the spread of
E. canis among a group
Minimize human exposure to
Safety monitoring of biological products and vaccines
that derive from susceptible animals
requirement: 0.2 ml whole blood in EDTA (purple top) tube, or
0.2 ml synovial fluid, or tick.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen
types, if there will be a delay in shipping, or during very warm
weather, refrigerate specimens until shipped and ship with a cold pack
unless more stringent shipping requirements are specified. Frozen
specimens should be shipped so as to remain frozen in transit. See
shipping instructions for more
2 business days
Qualitative real time