Canine adenovirus 2 PCR test
dog and cat assay data sheet
adenovirus type 2 (CAV-2)
Ultrasensitive qualitative detection of canine adenovirus
type 2 by real time polymerase chain reaction
S0103 is included
on P0019 - canine respiratory
linear, double-stranded DNA viruses which infect a wide variety of
mammals and birds. Two adenoviruses have been identified in the dog:
canine adenovirus type 1 (CAV-1) which infects most of the major
organs causing, among other diseases, hepatitis; and canine adenovirus
type 2 (CAV-2) which causes respiratory and enteric diseases.
CAV-2 is one of
the major causes of infectious tracheobronchitis in dogs, also known
as kennel cough. Vaccination against CAV-2 will not prevent infection
with this virus but it can minimize its severity so that the chances
of developing secondary bacterial infections and complications are
of kennel cough will develop within a week after a dog has been
exposed to CAV-2. The most common symptoms are a dry, hacking cough
followed by retching, and coughing up a white foamy discharge. The
cough is brought on by an inflammation of the trachea and bronchi.
Some dogs also develop conjunctivitis, rhinitis or a nasal discharge.
Diagnosis of CAV-2
has been done by electron microscopy, viral culture or serological
testing. However, these tests are laborious and do not have high
sensitivity. Molecular detection by PCR is a rapid, highly sensitive
and specific technique for detecting CAV-2.
Help confirm the disease causing agent
Help ensure that animal groups and populations are free of
canine adenovirus type 2
Early prevention of spread of CAV-2 among a population
Minimize human exposure to this virus
Safety monitoring of biological products and vaccines
that derive from susceptible animals
Nasopharyngeal, conjunctival or throat swab, or 0.2 ml tracheal wash,
or 0.2 ml feces, or rectal swab, or 0.2 ml fresh or frozen tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen
types, if there will be a delay in shipping, or during very warm
weather, refrigerate specimens until shipped and ship with a cold pack
unless more stringent shipping requirements are specified. Frozen
specimens should be shipped so as to remain frozen in transit. See
shipping instructions for more
2 business days
Qualitative real time PCR