Top dogs can catch things too!  Our NEW dog show panel checks for 8 pathogens potentially transmissible at dog shows.

 Neuro symptoms getting on your nerves? Try our canine neurological panel - 6 neurological pathogens from 1 CSF sample; or our feline neurological panel - 5 neurological pathogens from 1 CSF sample.

Oh baby! Try our canine breeding PCR panel - 3 canine sexually transmitted diseases tested from swabs or semen samples.

Respiratory symptoms got you breathless? Try our canine respiratory PCR panel - we test for 8 canine respiratory pathogens from throat, nasal and eye swabs.

...or maybe you need our feline respiratory PCR panel -- 6 feline respiratory pathogens from throat, nasal and eye swabs.

Diarrhea got you on the run? Try our canine diarrhea PCR panel -- 8 major diarrheagenic agents from 1 fecal specimen...
...OR our 9-pathogen feline diarrhea PCR panel.

Not feeling sanguine about bloodborne pathogens in cats? Try our feline bloodborne PCR panel -- 4 major bloodborne pathogens from 1 blood sample.

Ticks bugging you? Try our tickborne disease PCR panel -- 7 major tickborne pathogens from 1 blood sample.

Just plain sick and tired? Try our canine anemia PCR panel or our feline anemia PCR panel -- detect and differentiate multiple anemia pathogens from 1 blood sample.

            * * *           

Zoologix performs canine and feline PCR tests for...

Anaplasma phagocytophilum

Anaplasma platys

Aspergillus species

Aspergillus fumigatus

Babesia

Bartonella

Baylisascaris procyonis

Bordetella bronchiseptica

Borrelia burgdorferi

Brucella canis

Campylobacter

Canine adenovirus type 1

Canine adenovirus type 2

Canine circovirus

Canine enteric coronavirus (CCV1)

Canine distemper

Canine herpesvirus

Canine papillomavirus

Canine parainfluenza virus

Canine parvovirus

Canine pneumovirus

Canine respiratory coronavirus (CCV2)

Chagas disease

Chikungunya virus

Chlamydophila psittaci

Clostridium species

Coccidia

Cryptococcus

Cryptosporidium

Cytauxzoon felis

Demodex gatoi mites

E. coli

Ehrlichia

Entamoeba

Fading kitten syndrome

Feline calicivirus

Feline distemper

Feline enteric coronavirus

Feline foamy virus

Feline herpesvirus type 1

Feline immunodeficiency virus

Feline infectious anemia

Feline infectious peritonitis

Feline leukemia

Feline panleukopenia

Feline papillomavirus

Feline pneunomitis

Feline rhinotracheitis virus

Feline sarcoma virus

Feline syncytial virus

Francisella tularensis

Giardia

Group G strep

Haemobartonella canis

Haemobartonella felis

Helicobacter

Influenza

Lawsonia intracellularis

Leishmania

Leptospira

Lyme disease

Mange in cats

Microsporum

MRSA (Methicillin-resistant Staph aureus)

Mycoplasma canis

Mycoplasma cynos

Mycoplasma felis

Mycoplasma haemocanis

Mycoplasma haemofelis

Neorickettsia helmintheca

Neospora caninum

Pasteurella multocida

Pneumocystis carinii

Rabies

Reovirus screen

Rickettsia screen

Ringworm

Salmonella

Salmon poisoning disease

Sarcocystis neurona

Streptococcus, Group G

Streptococcus pneumoniae

Streptococcus pyogenes

Streptococcus zooepidemicus

Toxoplasma gondii

Trichomonas/
Tritrichomonas

Trichophyton

Trypanosoma cruzi

Tularemia

West Nile virus

Yersinia pestis

Yersinia pseudotuberculosis


Canine pneumovirus PCR test

dog and cat assay data sheet

Canine pneumovirus (CnPnV)

Test code:
S0236 - Ultrasensitive qualitative detection of canine pneumovirus by reverse transcription real time polymerase chain reaction

Canine pneumovirus (CnPnV) is an enveloped, single-strand negative-sense RNA virus associated with respiratory disease in dogs. It is considered one of the etiological agents of canine infectious respiratory disease complex (CIRDC), also known as “kennel cough” or “canine shipping fever.”

CnPnV belongs to family Paramyxoviridae, subfamily Pneumovirinae, genus Orthopneumovirus, This genus includes viruses associated with both animal and human respiratory pathogens, such as human respiratory syncytial viruses (HRSV), bovine respiratory syncytial virus (BRSV), murine pneumonia virus (MPV) and the recently discovered novel swine orthopneumovirus (SOV).  Complete genome sequence analysis of CPV shows that it is closely related to mouse pneumonia virus (MPV), with 95–96% nucleotide identity.

CnPnV was discovered in 2010 and subsequent investigations into the prevalence of CPV in respiratory illness dogs showed widespread occurrence in various outbreaks among canine breeding colonies and shelters in the United States and Europe. Furthermore, retrospective surveys of CPV seroprevalence in European dogs revealed an estimated CPV-seropositive level of about 50% in pet dogs and a markedly higher level of up to 93.5% in kenneled dogs. Thus, CPV is widely spread over various areas in America and Europe and is not geographically limited.

Similar to other pathogens in the CIRDC, transmission of this virus is mainly through aerosolized droplets; or direct contact with saliva or nasal secretions of infected dogs. The virus initially colonizes the respiratory epithelium lining the nasal cavity, trachea, and bronchi. Because most dogs are infected through exposure to aerosolized secretions, there is often a predictable temporal relationship between exposure to other dogs and the onset of clinical symptoms, anywhere from 3-10 days in most cases.

Diagnosis of canine pneumovirus requires the use of molecular detection techniques such as polymerase chain reaction (PCR), because it is very difficult to culture this virus (Mitchell et al., 2013).

Utilities:

  • Help confirm the disease causing agent
  • Help ensure that animal groups and populations are free of canine pneumovirus
  • Early prevention of spread of this virus among a population
  • Minimize human exposure to this virus
  • Safety monitoring of biological products and vaccines that derive from susceptible animals

References:
Mitchell, J. A., Cardwell, J. M., Renshaw, R. W., Dubovi, E. J., & Brownlie, J. (2013). Detection of canine pneumovirus in dogs with canine infectious respiratory disease. Journal of clinical microbiology, 51: 4112–4119.

Specimen requirement: Nasal swab, or pharyngeal swab, or 0.2 ml tracheal wash or bronchoalveolar lavage, or 0.2 ml fresh or frozen tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription real time PCR

Normal range: Nondetected

2003-2020 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880