X0002 - Ultrasensitive qualitative detection of
Toxoplasma gondii by real time polymerase chain reaction
by Toxoplasma gondii
are prevalent in many species of domestic and wild animals (Dubey,
1993). Although chronic infection of this obligate parasite is
usually asymptomatic, T. gondii has emerged as a major
opportunistic pathogen in the immunocompromised, such as AIDS
patients. Surprisingly, spontaneous cases of fulminating fatal
toxoplasmosis have been reported in adult squirrel monkeys (Saimiri
sciureus) which are immunologically normal (Inoue, 1997). While
the disease mechanism of this fatal toxoplasmosis in squirrel
monkeys is not clear, infections of nonhuman primates with this
parasite are common, and it has been shown that New World
monkeys are more susceptible to T. gondii infection than Old
World monkeys (Ruch, 1959). The outbreak of fatal toxoplasmosis
(Cunningham, et al., 1992) is a major concern in caring for
these primate colonies, as a recent study has indicated the
possibility of horizontal transmission through the respiratory
route (Furuta, et al., 2001) in addition to the fecal-oral
standard” for the detection of
T. gondii in
clinical specimens is mouse inoculation and detection of
T. gondii specific
antibodies. This method is sensitive and specific but very
time-consuming, taking up to six weeks to obtain a diagnosis.
Cell culture detection of this parasite is also slow, and lacks
sensitivity. PCR detection of this parasite has been found to be
a sensitive, specific and rapid method for the detection of
T. gondii DNA in a
wide spectrum of samples, such as amniotic fluid (Grover, et
al., 1990), blood (Dupouy-Camet, et al., 1993; Ho-Yen, et al.,
1992), tissue samples (Johnson, et al., 1993) and cerebrospinal
fluid (Cristina, et al., 1993; Farmley, et al., 1992). Although
serology testing can help diagnose recent infection with this
parasite, PCR testing is found to be more sensitive in
identifying acute infection (Hussein, et al., 2002).
Help confirm the disease causing agent
Help ensure that animal colonies are free of
Early prevention of spread of this parasite among a
Minimize personnel exposure to this parasite
Safety monitoring of biological products and vaccines
that derive from primates
Dubey, J.P. (1993) Toxoplasma, Neoplasma, Sarcocystis, and
other tissue cyst-forming coccidian of human and animals.
pp1-56. In: Parasitic protozoa (Kreier, P.J. ed), vol. 6, 2nd
ed., Academic Press, Inc., San Diego, California.
(1997) Acute toxoplasmosis in squirrel monkeys. J. Vet. Med.
Ruch, T.C. (1959). pp.297-299, 313-318,
423-424. In: Diseases of laboratory primates. W.B. Saunders Co.,
Cunningham, A.A., Buxton, D. and Thomson, K.M.
(1992) An epidemic of toxoplasmosis in a captive colony of
squirrel monkeys (Saimiri sciureus). J. Comp. Pathol.
Furuta, T., Une, Y., Omura, M., Matsutani, N.,
Nomura, Y., Kikuchi, T., Hattori, S. and Yoshikawa, Y. (2001)
Horizontal transmission of Toxoplasma gondii in squirrel monkeys
(Saimiri sciureus). Exp. Anim. 50:299-306.
Thulliez, P., Remington, J.S. and Boothroyd, J.C. (1990) Rapid
prenatal diagnosis of congenital Toxoplasma infection by using
polymerase chain reaction and amniotic fluid. J. Clin. Microbiol.
Dupouy-Camet, J., de Souza, S.L., Maslo, C.,
Paugam, A., Saimot, A.G., Benarous, R., Tourte-Schaefer, C. and
Derouin, F. (1993) Detection of Toxoplasma gondii in venous
blood from AIDS patients by polymerase chain reaction. J. Clin.
Ho-Yen, D.O., Joss, A.W.L., Balflour,
A.H., Smyth, E.T.M., Baird, D. and Chatterton, J.M.W. (1992) Use
of the polymerase chain reaction to detect Toxoplasma gondii in
human blood samples. J. Clin. Pathol. 45:910-913.
J.D., Butcher, P.D., Savva, D. and Holliman, R.E. (1993)
Application of the polymerase chain reaction to the diagnosis of
human toxoplasmosis. J. Infect. 26:147-158.
Pelloux, C., Goulhot, J.P., Brion, P., Leclercq, P. and Ambrosis-Thomas,
P. (1993) Detection of Toxoplasma gondii in AIDS patients by the
polymerase chain reaction. Infection 21:150-153.
S.F., Goebel, F.D. and Remington, J.S. (1992) Detection of
Toxoplasma gondii in cerebrospinal fluid from AIDS patients by
polymerase chain reaction. J. Clin. Microbiol. 30:3000-3002.
Hussein, A.H., Nagaty, I.M. and Fouad, M.A. (2002) Evaluation of
IgM-ELISA versus PCR in diagnosis of recent Toxoplasma gondii
infection. J Egypt Soc Parasitol. 32:639-46.
Specimen requirement: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top)
tube, or 0.2 ml feces, or 0.2 ml amniotic fluid or CSF, or 0.2 ml fresh, frozen or fixed tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
real time PCR