primate assay data sheet
Cynomolgus polyomavirus
Test code:
S0257
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Ultrasensitive qualitative detection of cynomolgus polyomavirus by real
time PCR.
Polyomaviruses are double-stranded DNA viruses that belong to the
family Papovaviridae.
Papillomaviruses are also in this family. Humans and animals
infected by polyomaviruses usually do not develop major
life-threatening clinical symptoms, so carriers of these viruses
may not be easily detected. However, in immune-compromised
individuals these polyomaviruses can cause significant damage to
organs including brain, kidney and liver.
Polyomaviruses have a narrow host range, so it is not common for a
polyomavirus from one primate species to infect another species.
Simian virus 40 (SV40) is a polyomavirus which which infects
rhesus monkeys and is very similar in genomic sequence to
cynomolgus polyomavirus. However, there are significant
differences between the two viruses in protein composition,
leading to the conclusion that cynomolgus polyomavirus is a
separate viral species which is distinct from SV40.
Cynomolgus polyomavirus seroprevalence in captive and wild cynomolgus
monkeys is not known. However, in a study (Verschoor et al.,
2008) measuring antibodies that cross-react with SV40 in
colonies of captive monkeys, high seropositive rates were found
in rhesus macaques (74.7%), cynomolgus macaques (44.8%) and
Tonkean macaques (41.7%), especially in animals imported from
China. Much lower seropositive rates were detected in cynomolgus
macaques from Mauritius (8.8%). Monkeys that have been exposed
to cynomolgus polyomavirus may potentially be carriers, with
reactivation occurring under stress or clinical treatments
impacting the immune system.
It is difficult to diagnose carrier status using serological tests.
Molecular detection by polymerase chain reaction is an easy,
quick and specific way to identify the presence of this virus.
Utilities:
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Help confirm the disease causing agent
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Help ensure that animal colonies are free of this virus
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Early prevention of spread of this virus among a colony
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Minimize personnel exposure to this virus
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Safety monitoring of biological products and vaccines that
derive from primates
References:
Verschoor, E.J., Niphuis, H., Fagrouch, Z., Christian, P.,
Sasnauskas, K., Pizarro, M.C. and Heeney, J.L. (2008)
Seroprevalence of SV40-like polyomavirus infections in captive
and free-ranging macaque species. J. Med. Primatol.
37:196-201.
Specimen requirement: 0.2 ml
fresh tissue, or 0.2 ml EDTA whole blood, or 0.2 ml urine, or 0.2 ml
frozen or fixed tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a
delay in shipping, or during very warm weather, refrigerate
specimens until shipped and ship with a cold pack unless more
stringent shipping requirements are specified. Frozen
specimens should be shipped so as to remain frozen in transit.
See shipping instructions for
more information.
Turnaround time: 2 business
days
Methodology:
Ultrasensitive qualitative
real time PCR
Normal range: Nondetected