Vesicular stomatitis virus (VSV) NOTE: THIS TEST IS NOT PERFORMED ON SAMPLES TAKEN FROM ANIMALS OWNED OR LOCATED IN THE STATE OF CALIFORNIA.

Test code: S0068 - Ultrasensitive qualitative detection and differentiation of the New Jersey and Indiana serotypes of vesicular stomatitis virus by reverse transcription coupled real time polymerase chain reaction.

Vesicular stomatitis (VS) is a disease of horses, cattle, swine, sheep, goats and deer caused by related viruses in the genus Vesiculovirus of the family Rhabdoviridae. The viral genome is 11 kb and codes for five main proteins: the nucleocapsid (N), phosphoprotein (P), matrix (M), glycoprotein(G), and polymerase (L). Serologically, VS viruses have been classified into two main serotypes: New Jersey (NJ) and Indiana (IN). In addition to the classic VSV-IN (IN1), two additional subtypes, Cocal virus (VSV-IN2) and Alagoas virus (VSV-IN3), have been described.

VS is prevalent in northern South America, throughout Central America, and at one site in the United States: Ossabaw Island, Georgia. However, outbreaks of VS have been reported in the western United States, northern Mexico, and temperate regions of South America. The disease can cause blisters on an animal's mouth, teats or hooves. Clinical outbreaks of VS in livestock can result in significant economic impact on producers and extensive regulatory responses by governmental agencies, including trade restrictions, market closures, and quarantines. Such measures are needed because of the clinical similarities between VS and foot-and-mouth disease and other foreign vesicular diseases in livestock species.

Utilities:

• Help confirm the disease causing agent
• Distinguish between VS and other vesicular diseases including foot-and-mouth
• Shorten the time required to confirm a clinical diagnosis of VS infection.
• Help ensure that herds are free of VS
• Early prevention of spread of this virus
• Minimize personnel exposure to this virus
• Safety monitoring of biological products that derive from horses

References:
Magnuson, R.J., Triantis, J., Rodriguez, L.L., Perkins, A., Meredith, C.O., Beaty, B., McCluskey, B., and Salman M. (2003) A single-tube multiplex reverse transcription-polymerase chain reaction for detection and differentiation of vesicular stomatitis Indiana 1 and New Jersey viruses in insects. J. Vet. Diagn. Invest. 15:561-567.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) tube, or lesion swab or nasopharyngeal swab, or 0.2 ml nasal wash, or 0.2 ml fresh or frozen tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected