Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

For wild rodent infestations, remediation and environmental monitoring, use our Rodent Infestation PCR Panel

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

COVID19

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Leptospira

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit coronavirus

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi/ paraluiscuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


PCR test for COVID19 in rodents

rodent and rabbit assay data sheet

COVID-19 (aka "novel coronavirus", 2019-nCoV, SARS-CoV-2)

Test code:
S0235 - Ultrasensitive qualitative detection of COVID19 by one-step reverse transcription real time PCR.

CDC guidance on SARS-CoV-2 testing in animals:

  • The decision to test an animal (including research animals, companion animals, livestock, and wild or zoo animals) should be based upon a One Health approach. Consultation between veterinarians and appropriate local, state, and/or federal public health and animal health officials is recommended.

  • USDA-NVSL can perform confirmatory testing on any samples initially tested positive at Zoologix, and reports any confirmed positive cases to the appropriate agencies to track the disease.

  • Other more common causes of illness in animals should be considered before considering SARS-CoV-2 testing.

Zoologix performs COVID-19 PCR testing of research animal samples, and on environmental samples. We believe that the current shortage of human COVID-19 testing capacity can be mitigated by diverting non-human samples requiring COVID-19 testing away from human diagnostic labs whose full COVID-19 testing capacity is needed for processing human clinical samples.

For this testing we are using the same PCR primer set, which targets the same viral genetic sequence, as the US Centers for Disease Control (CDC)-specified test being used for human COVID-19 PCR testing. ZOOLOGIX DOES NOT PERFORM TESTING ON HUMAN CLINICAL SAMPLES. Zoologix performs this testing on animal samples submitted by veterinarians, and on environmental samples submitted by professional environmental firms.

The CDC is encouraging and enabling a broad-based, decentralized approach to COVID-19 testing. By performing this testing on research animal samples from research facilities and on environmental samples, Zoologix hopes to be able to support and contribute to this approach and do our part in helping to control the current outbreak.

The novel coronavirus (2019-nCoV or SARS-CoV-2) outbreak, which initially began in China, has spread rapidly around the globe. This novel coronavirus, first known as 2019-nCoV, has been officially named by the World Health Organization as COVID-19.

Various coronaviruses are found in animals and humans. Infection by these viruses usually results in respiratory and enteric symptoms. Historically, most coronaviral infections caused relatively mild human clinical symptoms, until the severe acute respiratory syndrome (SARS-CoV) outbreak in 2002 and 2003 in China, which captured the attention of the medical community regarding the severity of animal to human transmission of coronaviruses. A decade later, the Middle East respiratory syndrome coronavirus (MERS-CoV), another pathogenic coronavirus with a clinical presentation reminiscent of SARS, was isolated in human patients presenting with pneumonia. The MERS-CoV virus was again confirmed to be transmitted from animals (in that case camels) to human.

Similar to the origin of the HIV virus, scientists believe that when coronaviruses, which often have minimal effects on host animals, jump to humans, the human immune system may not be able to adapt and hence, humans may develop severe reactions to these viral infections. While the origin of the COVID-19 virus is not certain, scientists are inclined to believe that this virus derived from one of the exotic animals being sold for human consumption in a Chinese “wet market.”

Though much remains to learn about the transmission characteristics of this virus, including the range of animal species that can be infected, it appears that the virus can survive on environmental surfaces for some time and can infect rhesus macaques (Callaway, 2020).  Therefore to help protect people and laboratory animals from exposure, it may be useful to test swabs of these surfaces and research animals, including macaques and humanized mice, for the virus. PCR detection of the virus can also be used in ongoing basic research on the virus, as well as vaccine and therapeutic discovery work with mice, monkeys and other animals.

Molecular detection by PCR is currently the best approach to quickly identify animal carriers and environmental presence of the virus.  Due to the wide spread of the virus among humans, routine checking for the virus in animals having had contact with human caretakers may be necessary. Testing of surface swipes or swabs for the virus may also help control environmental transmission of the virus.

The primer sequence used in this test is identical to the primer sequence published by CDC.

Utilities:

  • THIS TEST IS NOT TO BE USED FOR HUMAN CLINICAL TESTING. IT IS FOR ANIMAL SAMPLES SUBMITTED BY A VETERINARIAN AND ENVIRONMENTAL SAMPLES ONLY.
  • Help identify research animals infected with the virus
  • Help prevent transmission of the virus on environmental surfaces
  • Minimize human exposure to the virus
  • Minimize unintended animal exposure to the virus
  • Virus research involving rodents and other research animals

References:
Lan, L., Xu, D., Ye, G., et al. (2020) Positive RT-PCR test results in patients recovered from COVID-19. JAMA. Published online February 27, 2020. doi:10.1001/jama.2020.2783.

Kampf, G., Todt, D., Pfaender, S., Steinmann, E. (March 2020) Persistence of coronaviruses on inanimate surfaces and their inactivation with biocidal agents, The Journal of Hospital Infection, Volume 104, Issue 3, Pages 246–251.

Callaway, Ewen (2020) Monkeys and Mice Enlisted to Fight Coronavirus. Nature, Volume 579, 12 March 2020, page 183.

Specimen requirements:  Nasal swab, or oral swab, or surface swab, or surface gauze pad swipe, or fecal pellet, or 0.1 ml EDTA whole blood (liquid or on dry card), or 0.1 ml fresh or frozen tissue. Ship fresh samples immediately, overnight, on cold paks. Ship frozen samples so as to remain frozen until their arrival at Zoologix.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

Turnaround time: 2 business days

Methodology: Qualitative one-step reverse transcription real time PCR

Normal range: Nondetected

2003-2020 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880