ELISA screening for diseases of nonhuman primates
- Simian retrovirus (SRV) ELISA. Serology screen detects
antibodies to serogroups 1, 2, 3, 4 and 5 group D SRV in
- Herpes B ELISA. Serology assay detects antibodies to herpes B
virus in macaques.
- Simian immunodeficiency virus (SIV) ELISA. Serology assay
detects antibodies to SIV in macaques.
- Simian T-cell leukemia virus (STLV) types 1 and 2 ELISA.
Serology screen detects antibodies to STLV types 1 and 2 in
- Measles ELISA. Serology assay detects antibodies to measles
virus in macaques.
- Hepatitis A total antibody detection by ELISA in nonhuman
- Hepatitis B total antibody detection by ELISA in nonhuman
- Hepatitis C total antibody detection by ELISA in nonhuman
Zoologix also performs
PCR tests for all the above pathogens.
and improve the quality of nonhuman primates used in biomedical
research, and to protect occupational safety and animal health,
the establishment and maintenance of specific pathogen free
(SPF) breeding colonies of macaques (genus Macaca) has always
been a priority. Four pathogens in particular are often targeted
for screening in NHP breeding programs: SRV, SIV, STLV and
Herpes B. Repeated screening by immunoassay is normally
performed for each of these, along with repeated PCR screening
for SRV. PCR is also used as a confirmatory or adjunct test for
SIV, STLV and Herpes B.
D retrovirus (SRV) is enzootic in many Asian macaque
populations, where it is associated with immunodeficiency
diseases. Many infected monkeys are asymptomatic carriers of the
virus. The virus can cause animal fatalities and confound
preclinical trials and other experimental protocols.
simian retroviruses, simian immunodeficiency virus (SIV) and
simian T-cell leukemia/lymphotropic virus (STLV), are also
commonly screened in SPF monkey colonies. Like SRV, these
viruses can significantly compromise the primate immune system
and cause fatal outcomes in monkeys subject to experimentation.
and specific screening tests can identify macaques infected with
exogenous SIV, STLV, and SRV. A testing algorithm of repeated
antibody screening by enzyme immunoassay plus molecular
detection by PCR has proven useful for identification and
exclusion of these retroviruses from monkey colonies (Buchl et
al., 1997; Lerche et al., 1994, 1997)
virus, which is widespread in many macaque populations, is a
significant health threat to primate care personnel. The virus
is normally asymptomatic in macaques, but human infection with
this virus is often fatal. Monkeys immunocompromised by
experimental retrovirus infection and drug treatment are more
likely to become B virus shedders. Although B virus is the most
challenging infection to eliminate from an SPF population,
active screening by serological testing plus molecular detection
in suspicious cases can help to eliminate the virus from
colonies. (Buchl et al., 1997).
Help confirm the disease causing agent
Help ensure that animal colonies are free of these viruses
Early prevention of spread of these viruses among a
Minimize personnel exposure to these viruses
Elimination of confounding infections from experimental
Safety monitoring of biological products and vaccines
that derive from primates
Lerche, N.W. Yee, J.L. and Jennings, M.B. (1994) Establishing
specific retrovirus-free breeding colonies of macaques: an
approach to primary screening and surveillance. Lab Anim Sci.
Lerche, N.W., Cotterman, R.F., Dobson, M.D., Yee,
J.L., Rosenthal, A.N. and Heneine, W.M. (1997) Screening for
simian type-D retrovirus infection in macaques, using nested
polymerase chain reaction. Lab Anim Sci. 47:263-268.
S.J., Keeling, M.E., Voss, W.R. (1997) Establishing Specific
Pathogen-Free (SPF) Nonhuman Primate Colonies. ILAR J. 38:22-27.
Specimen requirements: 0.5 ml whole blood in EDTA (purple top) tube
is adequate volume for up to 8 different ELISA tests.
NOTE: DO NOT send serum, plasma, or serum separator
tubes for any ELISA assay. Zoologix performs
serum separation from EDTA whole blood as part of our ELISA
testing service, at no extra charge.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
detection by Enzyme-Linked ImmunoSorbent Assay