Zoologix performs primate infectious disease tests by PCR for...

Adenoviruses

African green monkey endogenous virus

Aspergillus

B virus

Babesia

Baboon endogenous virus

Baylisascaris procyonis

Borrelia burgdorferi

Burkholderia

Campylobacter

Chagas' disease

Chikungunya virus

Chlamydia pneumoniae

Chlamydophila trachomatis

Clostridium

Coccidioides

Cronobacter sakazakii

Cryptosporidium

Cytomegalovirus, baboon

Cytomegalovirus, chimpanzee

Cytomegalovirus, human

Cytomegalovirus, macaque

Cytomegalovirus, simian

Dengue

E. coli O157:H7

E. coli panel

Encephalitis, Japanese

Encephalitis, St. Louis

Encephalomyocarditis (EMCV)

Entamoeba species

Enterovirus

Epstein-Barr virus

Giardia

Gibbon ape leukemia

Helicobacter

Hepatitis A virus

Hepatitis B virus

Hepatitis C virus

Herpes ateles

Herpes B virus

Herpes simplex type 1

Herpes simplex type 2

Herpes tamarinus

Herpesvirus ateles

Herpesvirus papio 1 & 2

Herpesvirus saimiri

Human adenoviruses

Human herpesviruses types 6, 7 & 8

Human immunodeficiency virus types 1 & 2

Human T cell lymphotropic virus

Human Varicella-Zoster

Influenza

Klebsiella

Lawsonia intracellularis

Leishmania

Leptospira

Lyme disease

Lymphocryptovirus

Malaria

Measles

Monkeypox

Mycobacteria

Mycoplasma

Neisseria gonorhoeae

Neisseria meningitidis

Papillomavirus

Parvoviruses

Plasmodium species

Reovirus screen

Rhesus rhadinovirus

Rotavirus

Salmonella

SARS-CoV-2

Shigella and enteroinvasive E. coli

Simian agent 6 (SA6)

Simian agent 8 (SA8)

Simian foamy virus (SFV)

Simian hemorrhagic fever (SHFV)

Simian immunodeficiency virus (SIV)

Simian parainfluenza virus

Simian retrovirus (SRV)

Simian sarcoma virus

Simian T-cell leukemia (STLV) types 1 & 2

Simian T-cell leukemia (STLV) type 3

Simian Varicella-Zoster

Squirrel monkey retrovirus

Streptococcus pneumoniae

Streptococcus pyogenes

SV40

SV5

Syphilis

Tetanus

Toxoplasma gondii

Treponema

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tuberculosis

Ureaplasma

Valley fever

West Nile virus (WNV)

Yaws

Yellow fever

Yersinia pestis

Yersinia pseudotuberculosis

Zika virus

* * *

Genetic tests for...

A/B/AB blood type in macaques

Cynomolgus genotyping

Fetal sexing

Mamu-6 in macaques

Mamu-7 in macaques

CYP2C76 c.449TG>A
in macaques

Mu opioid receptor
in macaques

smCCR5Δ24
in sooty mangabeys

...and more - contact Zoologix with your genetic testing requirements


SARS-CoV-2 PCR test for non-human primates

primate assay data sheet

SARS-CoV-2 (aka "novel coronavirus", 2019-nCoV, COVID-19)

Test code:
S0235 - Ultrasensitive qualitative detection of SARS-CoV-2 by one-step reverse transcription real time PCR.

CDC guidance on SARS-CoV-2 testing in animals:

  • The decision to test an animal (including research animals, companion animals, livestock, and wild or zoo animals) should be based upon a One Health approach. Consultation between veterinarians and appropriate local, state, and/or federal public health and animal health officials is required.

  • USDA-NVSL will perform confirmatory testing on any samples initially tested positive at Zoologix, and reports any confirmed positive cases to the appropriate agencies to track the disease.

  • Other more common causes of illness in animals should be considered before considering SARS-CoV-2 testing.

Zoologix performs SRS-CoV-2 PCR testing on environmental samples submitted to us by professional environmental firms, and on animal samples submitted to us by a veteriarian following applicable guidelines within their jurisdiction. Human samples will not be accepted.

The novel coronavirus (2019-nCoV or SARS-CoV-2) outbreak, which initially began in China, has spread rapidly around the globe. The disease caused by this novel coronavirus, first known as 2019-nCoV, was officially named by the World Health Organization as COVID-19.

Various coronaviruses are found in animals and humans. Infection by these viruses usually results in respiratory and enteric symptoms. Historically, most coronaviral infections caused relatively mild human clinical symptoms, until the severe acute respiratory syndrome (SARS-CoV) outbreak in 2002 and 2003 in China, which captured the attention of the medical community regarding the severity of animal to human transmission of coronaviruses. A decade later, the Middle East respiratory syndrome coronavirus (MERS-CoV), another pathogenic coronavirus with a clinical presentation reminiscent of SARS, was isolated in human patients presenting with pneumonia. The MERS-CoV virus was again confirmed to be transmitted from animals (in that case camels) to human.

Similar to the origin of the HIV virus, scientists believe that when coronaviruses, which often have minimal effects on host animals, jump to humans, the human immune system may not be able to adapt and hence, humans may develop severe reactions to these viral infections. While the origin of the COVID-19 virus is not certain, scientists are inclined to believe that this virus derived from one of the exotic animals being sold for human consumption in a Chinese “wet market.”

Though much remains to learn about the transmission characteristics of this virus, including the range of animal species that can be infected, it appears that the virus can survive on environmental surfaces for some time and can infect rhesus macaques (Callaway, 2020).  Therefore to help protect people and laboratory animals from exposure, it may be useful to test swabs of these surfaces and research animals, including macaques, for the virus. PCR detection of the virus can also be used in ongoing basic research on the virus, as well as vaccine and therapeutic discovery work with monkeys and other animals.

Molecular detection by PCR is currently the best approach to quickly identify animal carriers and environmental presence of the virus.  Due to the wide spread of the virus among humans, routine checking for the virus in animals having had contact with human caretakers may be necessary. Testing of surface swipes or swabs for the virus may also help control environmental transmission of the virus.

The primer sequence used in this test is identical to the primer sequence published by CDC.

Utilities:

  • THIS TEST IS ONLY TO BE USED FOR ENVIRONMENTAL SAMPLES, OR FOR ANIMAL SAMPLES SUBMITTED BY A VETERINARIAN PER THE GUIDELINES MENTIONED ABOVE.
  • Help identify research animals infected with the virus
  • Help prevent transmission of the virus on environmental surfaces
  • Minimize human exposure to the virus
  • Minimize unintended animal exposure to the virus
  • Virus research involving nonhuman primates and other research animals

References:
Lan, L., Xu, D., Ye, G., et al. (2020) Positive RT-PCR test results in patients recovered from COVID-19. JAMA. Published online February 27, 2020. doi:10.1001/jama.2020.2783.

Kampf, G., Todt, D., Pfaender, S., Steinmann, E. (March 2020) Persistence of coronaviruses on inanimate surfaces and their inactivation with biocidal agents, The Journal of Hospital Infection, Volume 104, Issue 3, Pages 246–251.

Callaway, Ewen (2020) Monkeys and Mice Enlisted to Fight Coronavirus. Nature, Volume 579, 12 March 2020, page 183.

Specimen requirements:  Nasal swab, or oral swab, or surface swab, or surface gauze pad swipe, or 0.2 ml feces or fecal swab, or 0.2 ml EDTA whole blood (liquid or on dry card), or 0.2 ml fresh or frozen tissue. Ship fresh samples immediately, overnight, on cold paks. Ship frozen samples so as to remain frozen until their arrival at Zoologix.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

Turnaround time: 2 business days

Methodology: Qualitative one-step reverse transcription real time PCR

Normal range: Nondetected

 
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