Moving reptiles?  Use our snake and lizard quarantine PCR panel to avoid spreading contagious agents.

Ruminating about hoofstock issues?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

Our Rodent Infestation PCR Panel tests for 5 common pathogens found in rodent-contaminated facilities.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Anisakis worms

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Camelpox

Campylobacter

Canine circovirus

Canine distemper

Canine parvovirus

Capillaria xenopodis

Chlamydia/
Chlamydophila

Chlamydophila pneumoniae

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coccidioides

Coronaviruses

Coxiella burnetii

Cryptococcosis

Cryptosporidium

Cryptosporidium serpentis

Cryptosporidium varanii (formerly saurophilum)

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Hantavirus

Helicobacter

Hepatitis E

Herring worms

Histoplasma

Influenza

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leishmania

Leptospira

Listeria monocytogenes

Lizard quarantine panel

Lyme disease

Macropodid (kangaroo) herpesviruses

Malaria

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Ophidiomyces ophiodiicola

Pasteurella multocida

Plasmodium species

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudocapillaroides xenopi

Pseudoloma neurophilia

Pseudorabies

Pseudoterranova worms

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

SARS-CoV-2

Snake fungal disease

Snake quarantine panel

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Valley Fever

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


SARS-CoV-2 PCR test for animals and environmental surfaces

environmental and zoo assay data sheet

SARS-CoV-2 (aka "novel coronavirus", 2019-nCoV, COVID-19)

Test code:
S0235 - Ultrasensitive qualitative detection of SARS-CoV-2 by one-step reverse transcription real time PCR.

CDC guidance on SARS-CoV-2 testing in animals:

  • The decision to test an animal (including research animals, companion animals, livestock, and wild or zoo animals) should be based upon a One Health approach. Consultation between veterinarians and appropriate local, state, and/or federal public health and animal health officials is required.

  • USDA-NVSL will perform confirmatory testing on any samples initially tested positive at Zoologix, and reports any confirmed positive cases to the appropriate agencies to track the disease.

  • Other more common causes of illness in animals should be considered before considering SARS-CoV-2 testing.

Zoologix performs SRS-CoV-2 PCR testing on environmental samples submitted to us by professional environmental firms, and on animal samples submitted to us by a veteriarian following applicable guidelines within their jurisdiction. Human samples will not be accepted.

The novel coronavirus (2019-nCoV or SARS-CoV-2) outbreak, which initially began in China, has spread rapidly around the globe. The disease caused by this novel coronavirus, first known as 2019-nCoV, was officially named by the World Health Organization as COVID-19.

Various coronaviruses are found in animals and humans. Infection by these viruses usually results in respiratory and enteric symptoms. Historically, most coronaviral infections caused relatively mild human clinical symptoms, until the severe acute respiratory syndrome (SARS-CoV) outbreak in 2002 and 2003 in China, which captured the attention of the medical community regarding the severity of animal to human transmission of coronaviruses. A decade later, the Middle East respiratory syndrome coronavirus (MERS-CoV), another pathogenic coronavirus with a clinical presentation reminiscent of SARS, was isolated in human patients presenting with pneumonia. The MERS-CoV virus was again confirmed to be transmitted from animals (in that case camels) to human.

Similar to the origin of the HIV virus, scientists believe that when coronaviruses, which often have minimal effects on host animals, jump to humans, the human immune system may not be able to adapt and hence, humans may develop severe reactions to these viral infections. While the origin of the COVID-19 virus is not certain, some scientists are inclined to believe that this virus derived from one of the exotic animals being sold for human consumption in a Chinese “wet market.”

Though much remains to learn about the transmission characteristics of this virus, it appears that it can survive on environmental surfaces for some time.  Therefore to help protect people from exposure, it may be useful to test swabs of these surfaces for the virus.

It has been reported that SARS-CoV-2 replicates poorly in dogs, pigs, chickens and ducks, but efficiently in ferrets and cats. The virus transmits in cats via respiratory droplets (Shi 2020).

It was reported on 06 April 2020 that a tiger exhibiting symptoms consistent with COVID-19 in the Bronx Zoo has tested positive for the virus (New York Times).

Molecular detection by PCR is currently the best approach to quickly identify environmental presence of the virus. Testing of surface swipes or swabs for the virus may help control environmental transmission of the virus.

The primer sequence used in this test is identical to primer sequence published by CDC.

Utilities:

  • THIS TEST IS ONLY TO BE USED FOR ENVIRONMENTAL SAMPLES, OR FOR ANIMAL SAMPLES SUBMITTED BY A VETERINARIAN PER THE GUIDELINES MENTIONED ABOVE.
  • Detect the presence of COVID-19 virus on environmental surfaces
  • Minimize human and animal exposure to the virus

References:
Lan, L., Xu, D., Ye, G., et al. (2020) Positive RT-PCR test results in patients recovered from COVID-19. JAMA. Published online February 27, 2020. doi:10.1001/jama.2020.2783.

Shi, Jianzhong et al. (2020) Susceptibility of ferrets, cats, dogs, and different domestic animals to SARS-coronavirus-2. Preprint published March 30, 2020, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.

New York Times:  https://www.nytimes.com/2020/04/06/nyregion/bronx-zoo-tiger-coronavirus.html

Specimen requirements:  Surface gauze pad swipes or swabs; or nasopharyngeal swabs. Ship fresh samples immediately, overnight, on cold paks. Ship frozen samples so as to remain frozen until their arrival at Zoologix.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

Turnaround time: 2 business days

Methodology: Qualitative one-step reverse transcription real time PCR

Normal range: Nondetected

2003-2020 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880