Zoologix performs primate infectious disease tests by PCR for...


African green monkey endogenous virus


B virus


Baboon endogenous virus

Baylisascaris procyonis

Borrelia burgdorferi



Chagas' disease

Chikungunya virus

Chlamydia pneumoniae

Chlamydophila trachomatis



Cronobacter sakazakii


Cytomegalovirus, baboon

Cytomegalovirus, chimpanzee

Cytomegalovirus, human

Cytomegalovirus, macaque

Cytomegalovirus, simian


E. coli O157:H7

E. coli panel

Encephalitis, Japanese

Encephalitis, St. Louis

Encephalomyocarditis (EMCV)

Entamoeba species


Epstein-Barr virus


Gibbon ape leukemia


Hepatitis A virus

Hepatitis B virus

Hepatitis C virus

Herpes ateles

Herpes B virus

Herpes simplex type 1

Herpes simplex type 2

Herpes tamarinus

Herpesvirus ateles

Herpesvirus papio 1 & 2

Herpesvirus saimiri

Human adenoviruses

Human herpesviruses types 6, 7 & 8

Human immunodeficiency virus types 1 & 2

Human T cell lymphotropic virus

Human Varicella-Zoster



Lawsonia intracellularis



Lyme disease







Neisseria gonorhoeae

Neisseria meningitidis



Plasmodium species

Reovirus screen

Rhesus rhadinovirus



Shigella and enteroinvasive E. coli

Simian agent 6 (SA6)

Simian agent 8 (SA8)

Simian foamy virus (SFV)

Simian hemorrhagic fever (SHFV)

Simian immunodeficiency virus (SIV)

Simian parainfluenza virus

Simian retrovirus (SRV)

Simian sarcoma virus

Simian T-cell leukemia (STLV) types 1 & 2

Simian T-cell leukemia (STLV) type 3

Simian Varicella-Zoster

Squirrel monkey retrovirus

Streptococcus pneumoniae

Streptococcus pyogenes



Toxoplasma gondii

Treponema pallidum


Trypanosoma cruzi



Valley fever

West Nile virus (WNV)

Yellow fever

Yersinia pestis

Yersinia pseudotuberculosis

Zika virus

* * *

Genetic tests for...

A/B/AB blood type in macaques

Cynomolgus genotyping

Fetal sexing

Mamu-6 in macaques

Mamu-7 in macaques

CYP2C76 c.449TG>A
in macaques

Mu opioid receptor
in macaques

in sooty mangabeys

...and more - contact Zoologix with your genetic testing requirements

ELISA tests for primate screening
primate assay data sheet

ELISA screening for diseases of nonhuman primates

Test codes:

A0002 - Simian retrovirus (SRV) ELISA. Serology screen detects antibodies to serogroups 1, 2, 3, 4 and 5 group D SRV in macaques.
A0003 - Herpes B ELISA. Serology assay detects antibodies to herpes B virus in macaques.
A0004 - Simian immunodeficiency virus (SIV) ELISA. Serology assay detects antibodies to SIV in macaques.
A0005 - Simian T-cell leukemia virus (STLV) types 1 and 2 ELISA. Serology screen detects antibodies to STLV types 1 and 2 in macaques.
A0006 - Measles ELISA. Serology assay detects antibodies to measles virus in macaques.
A0007 - Hepatitis A total antibody detection by ELISA in nonhuman primates
A0008 - Hepatitis B total antibody detection by ELISA in nonhuman primates.
A0009 - Hepatitis C total antibody detection by ELISA in nonhuman primates.

Zoologix also performs PCR tests for all the above pathogens.

To maintain and improve the quality of nonhuman primates used in biomedical research, and to protect occupational safety and animal health, the establishment and maintenance of specific pathogen free (SPF) breeding colonies of macaques (genus Macaca) has always been a priority. Four pathogens in particular are often targeted for screening in NHP breeding programs: SRV, SIV, STLV and Herpes B. Repeated screening by immunoassay is normally performed for each of these, along with repeated PCR screening for SRV. PCR is also used as a confirmatory or adjunct test for SIV, STLV and Herpes B.

Simian type D retrovirus (SRV) is enzootic in many Asian macaque populations, where it is associated with immunodeficiency diseases. Many infected monkeys are asymptomatic carriers of the virus. The virus can cause animal fatalities and confound preclinical trials and other experimental protocols.

Two other simian retroviruses, simian immunodeficiency virus (SIV) and simian T-cell leukemia/lymphotropic virus (STLV), are also commonly screened in SPF monkey colonies. Like SRV, these viruses can significantly compromise the primate immune system and cause fatal outcomes in monkeys subject to experimentation.

Sensitive and specific screening tests can identify macaques infected with exogenous SIV, STLV, and SRV. A testing algorithm of repeated antibody screening by enzyme immunoassay plus molecular detection by PCR has proven useful for identification and exclusion of these retroviruses from monkey colonies (Buchl et al., 1997; Lerche et al., 1994, 1997)

Herpes B virus, which is widespread in many macaque populations, is a significant health threat to primate care personnel. The virus is normally asymptomatic in macaques, but human infection with this virus is often fatal. Monkeys immunocompromised by experimental retrovirus infection and drug treatment are more likely to become B virus shedders. Although B virus is the most challenging infection to eliminate from an SPF population, active screening by serological testing plus molecular detection in suspicious cases can help to eliminate the virus from colonies. (Buchl et al., 1997).


  • Help confirm the disease causing agent
  • Help ensure that animal colonies are free of these viruses
  • Early prevention of spread of these viruses among a colony
  • Minimize personnel exposure to these viruses
  • Elimination of confounding infections from experimental protocols
  • Safety monitoring of biological products and vaccines that derive from primates

Lerche, N.W. Yee, J.L. and Jennings, M.B. (1994) Establishing specific retrovirus-free breeding colonies of macaques: an approach to primary screening and surveillance. Lab Anim Sci. 44:217-221.
Lerche, N.W., Cotterman, R.F., Dobson, M.D., Yee, J.L., Rosenthal, A.N. and Heneine, W.M. (1997) Screening for simian type-D retrovirus infection in macaques, using nested polymerase chain reaction. Lab Anim Sci. 47:263-268.
Buchl, S.J., Keeling, M.E., Voss, W.R. (1997) Establishing Specific Pathogen-Free (SPF) Nonhuman Primate Colonies. ILAR J. 38:22-27.

Specimen requirements: 0.5 ml whole blood in EDTA (purple top) tube is adequate volume for up to 8 different ELISA tests.

NOTE: DO NOT send serum, plasma, or serum separator tubes for any ELISA assay.
  Zoologix performs serum separation from EDTA whole blood as part of our ELISA testing service, at no extra charge.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Antibody detection by Enzyme-Linked ImmunoSorbent Assay

Normal range: Nondetected

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