Need serology?
Yes, we're still the PCR experts. But Zoologix also performs ELISA antibody tests for...

SRV
Herpes B
SIV
STLV
Measles
Hepatitis A
Hepatitis B
Hepatitis C

* * *

Zoologix performs primate infectious disease tests by PCR for...

Adenoviruses

Aspergillus

B virus

Babesia

Baboon endogenous virus

Baylisascaris procyonis

Borrelia burgdorferi

Campylobacter

Chagas' disease

Chlamydia pneumoniae

Chlamydophila trachomatis

Clostridium

Cronobacter sakazakii

Cryptosporidium

Cytomegalovirus, baboon

Cytomegalovirus, chimpanzee

Cytomegalovirus, human

Cytomegalovirus, macaque

Cytomegalovirus, simian

Dengue

Ebola

E. coli O157:H7

E. coli panel

Encephalitis, Japanese

Encephalitis, St. Louis

Encephalomyocarditis (EMCV)

Entamoeba species

Enterovirus

Epstein-Barr virus

Filovirus panel

Giardia

Gibbon ape leukemia

Helicobacter

Hepatitis A virus

Hepatitis B virus

Hepatitis C virus

Herpes ateles

Herpes B virus

Herpes simplex type 1

Herpes simplex type 2

Herpes tamarinus

Herpesvirus ateles

Herpesvirus papio 1 & 2

Herpesvirus saimiri

Human adenoviruses

Human herpesviruses types 6, 7 & 8

Human immunodeficiency virus types 1 & 2

Human T cell lymphotropic virus

Human Varicella-Zoster

Influenza

Klebsiella

Lawsonia intracellularis

Leptospira

Lyme disease

Lymphocryptovirus

Malaria

Marburg virus

Measles

Monkeypox

Monkey parvoviruses

Mycobacteria

Mycoplasma

Neisseria gonorhoeae

Neisseria meningitidis

Papillomavirus

Plasmodium species

Reovirus screen

Rhesus rhadinovirus

Rotavirus

Salmonella

Shigella and enteroinvasive E. coli

Simian agent 6 (SA6)

Simian agent 8 (SA8)

Simian foamy virus (SFV)

Simian hemorrhagic fever (SHFV)

Simian immunodeficiency virus (SIV)

Simian parainfluenza virus

Simian retrovirus (SRV)

Simian sarcoma virus

Simian T-cell leukemia (STLV) types 1 & 2

Simian T-cell leukemia (STLV) type 3

Simian Varicella-Zoster

Squirrel monkey retrovirus

Streptococcus pneumoniae

Streptococcus pyogenes

SV40

SV5

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tuberculosis

Ureaplasma

West Nile virus (WNV)

Yellow fever

Yersinia pestis

Yersinia pseudotuberculosis

* * *

Genetic tests for...

A/B/AB blood type in macaques

Fetal sexing

Mamu-6 in macaques

Mamu-7 in macaques

CYP2C76 c.449TG>A
in macaques

Mu opioid receptor
in macaques

smCCR5Δ24
in sooty mangabeys

...and more - contact Zoologix with your genetic testing requirements


ELISA tests for primate screening
primate assay data sheet

ELISA screening for diseases of nonhuman primates

Test codes:

A0002 - Simian retrovirus (SRV) ELISA. Serology screen detects antibodies to serogroups 1, 2, 3, 4 and 5 group D SRV in macaques.
A0003 - Herpes B ELISA. Serology assay detects antibodies to herpes B virus in macaques.
A0004 - Simian immunodeficiency virus (SIV) ELISA. Serology assay detects antibodies to SIV in macaques.
A0005 - Simian T-cell leukemia virus (STLV) types 1 and 2 ELISA. Serology screen detects antibodies to STLV types 1 and 2 in macaques.
A0006 - Measles ELISA. Serology assay detects antibodies to measles virus in macaques.
A0007 - Hepatitis A total antibody detection by ELISA in nonhuman primates
A0008 - Hepatitis B total antibody detection by ELISA in nonhuman primates.
A0009 - Hepatitis C total antibody detection by ELISA in nonhuman primates.

Zoologix also performs PCR tests for all the above pathogens.

To maintain and improve the quality of nonhuman primates used in biomedical research, and to protect occupational safety and animal health, the establishment and maintenance of specific pathogen free (SPF) breeding colonies of macaques (genus Macaca) has always been a priority. Four pathogens in particular are often targeted for screening in NHP breeding programs: SRV, SIV, STLV and Herpes B. Repeated screening by immunoassay is normally performed for each of these, along with repeated PCR screening for SRV. PCR is also used as a confirmatory or adjunct test for SIV, STLV and Herpes B.

Simian type D retrovirus (SRV) is enzootic in many Asian macaque populations, where it is associated with immunodeficiency diseases. Many infected monkeys are asymptomatic carriers of the virus. The virus can cause animal fatalities and confound preclinical trials and other experimental protocols.

Two other simian retroviruses, simian immunodeficiency virus (SIV) and simian T-cell leukemia/lymphotropic virus (STLV), are also commonly screened in SPF monkey colonies. Like SRV, these viruses can significantly compromise the primate immune system and cause fatal outcomes in monkeys subject to experimentation.

Sensitive and specific screening tests can identify macaques infected with exogenous SIV, STLV, and SRV. A testing algorithm of repeated antibody screening by enzyme immunoassay plus molecular detection by PCR has proven useful for identification and exclusion of these retroviruses from monkey colonies (Buchl et al., 1997; Lerche et al., 1994, 1997)

Herpes B virus, which is widespread in many macaque populations, is a significant health threat to primate care personnel. The virus is normally asymptomatic in macaques, but human infection with this virus is often fatal. Monkeys immunocompromised by experimental retrovirus infection and drug treatment are more likely to become B virus shedders. Although B virus is the most challenging infection to eliminate from an SPF population, active screening by serological testing plus molecular detection in suspicious cases can help to eliminate the virus from colonies. (Buchl et al., 1997).

Utilities:

  • Help confirm the disease causing agent
  • Help ensure that animal colonies are free of these viruses
  • Early prevention of spread of these viruses among a colony
  • Minimize personnel exposure to these viruses
  • Elimination of confounding infections from experimental protocols
  • Safety monitoring of biological products and vaccines that derive from primates

References:
Lerche, N.W. Yee, J.L. and Jennings, M.B. (1994) Establishing specific retrovirus-free breeding colonies of macaques: an approach to primary screening and surveillance. Lab Anim Sci. 44:217-221.
Lerche, N.W., Cotterman, R.F., Dobson, M.D., Yee, J.L., Rosenthal, A.N. and Heneine, W.M. (1997) Screening for simian type-D retrovirus infection in macaques, using nested polymerase chain reaction. Lab Anim Sci. 47:263-268.
Buchl, S.J., Keeling, M.E., Voss, W.R. (1997) Establishing Specific Pathogen-Free (SPF) Nonhuman Primate Colonies. ILAR J. 38:22-27.

Specimen requirements: 0.5 ml whole blood in EDTA (purple top) tube is adequate volume for up to 8 different ELISA tests.

NOTE: DO NOT send serum, plasma, or serum separator tubes for any ELISA assay.
  Zoologix performs serum separation from EDTA whole blood as part of our ELISA testing service, at no extra charge.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Antibody detection by Enzyme-Linked ImmunoSorbent Assay

Normal range: Nondetected

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