dog and cat assay data sheet
Canine parainfluenza virus (CPIV)
Test code: S0109
- Ultrasensitive qualitative detection of canine
parainfluenza virus by reverse transcription real time
polymerase chain reaction
S0109 is included on
P0019
- Canine Respiratory Panel
Canine parainfluenza virus (CPIV) is an
important cause of kennel cough in the USA and is commonly
isolated from outbreaks of the disease. It has also been
identified in other parts of the world. Experimentally, the
disease is only very mild or subclinical. However, in the
field CPIV infection may predispose dogs to combined
infections with other viral and/or bacterial agents, leading
to more typical signs of kennel cough.
The virus replicates mainly in the
epithelial cells of the nasal mucosa, pharynx trachea, large
bronchi, and regional lymph nodes. Generalized infection does
not occur, except in immunocompromised animals.
CPIV can be detected by cell culture but
this method is not very sensitive. Molecular detection of PCR
is the most rapid, sensitive and specific method for
identifying this virus.
Utilities:
- Confirm the disease causing agent
- Ensure that animal groups and populations
are free of CPIV
- Early prevention of spread of CPIV among
a population
- Minimize human exposure to this virus
- Safety monitoring of biological products
and vaccines that derive from susceptible animals
Specimen requirement:
Nasopharyngeal swab or 1 ml tracheal wash, shipped overnight
at room temperature; or frozen tissue, shipped frozen.
For specimen types other than those listed
here, please call to confirm specimen acceptability and
shipping instructions.
For all specimen types, if there will be a
delay in shipping, or during very warm weather, refrigerate
specimens until shipped and ship with a cold pack unless more
stringent shipping requirements are specified. Frozen
specimens should be shipped so as to remain frozen in transit.
See shipping instructions for
more information.
Turnaround time: 2 business
days
Methodology: Qualitative
reverse transcription real time PCR
Normal range: Nondetected
