- Ultrasensitive detection of
Brucella bacteria by real time PCR. This assay detects but
does not differentiate
Brucella canis, B. abortus, B. microti, B. melitensis, B.
pinnipedialis, B. suis, B. ovis
and B. neotomae.
included in P0034 - canine breeding
Brucellosis is an infectious disease caused by bacteria of the genus
Many animal species are susceptible to
Some Brucella species
preferentially infect specific animal species. Humans can become
infected when they contact infected animals or their products.
caused by Brucella canis
is a sexually transmitted disease of dogs which can be spread during
is a major cause of canine infertility. It is primarily a sexually
transmitted disease, but can also be transmitted by contact with
infected bodily fluids. The disease can be passed from dogs to humans
In humans, brucellosis can cause a range of symptoms similar to
influenza, and may include fever, sweats, headaches, back pains, and
physical weakness. Severe infections of the central nervous system or
lining of the heart may occur. Brucellosis can also cause long-lasting
or chronic symptoms that include recurrent fevers, joint pain and
Although brucellosis is not common in United States, it frequently
occurs in countries that have poor animal disease control programs.
Travel to these countries can result in infection with these bacteria.
Humans are usually infected by eating or drinking something that is
contaminated with the bacteria, by inhaling the bacteria, or by
exposure of wounds to these bacteria. Occupational exposure is one of
the major routes for human infection. The chronic nature of the
disease means that infected people or animals can become carriers of
Diagnosis of brucellosis has traditionally been by culture detection
of the bacteria, but culture has very low sensitivity and so is less
useful for detection of subacute and chronic carriers. Molecular
detection by PCR, on the other hand, can provide rapid, sensitive and
specific identification of these bacteria in a sample (Mitka et al.,
Help confirm the disease causing agent
Help ensure that dog populations are free of
Early prevention of spread of
Brucella among dogs
Minimize human exposure to
Safety monitoring of biological products that derive
from susceptible animals
Mitka, S., Anetakis, C., Souliou, E., Diza, E. and Kansouzidou, A.
(2007). Evaluation of Different PCR Assays for Early Detection of
Acute and Relapsing Brucellosis in Humans in Comparison with
J. Clin. Microbiol.
0.2 ml fresh or frozen semen, or a vaginal swab, or a urethral swab,
or a prepuce swab, or 0.2 ml EDTA whole blood, or 0.2 ml milk.
For specimen types
other than those listed here, please call to confirm specimen
acceptability and shipping instructions.
For all specimen
types, if there will be a delay in shipping, or during very warm
weather, refrigerate specimens until shipped and ship with a cold pack
unless more stringent shipping requirements are specified. Frozen
specimens should be shipped so as to remain frozen in transit. See
shipping instructions for more
2 business days
Qualitative real time PCR